海洋冷渗中含氮生物的丰度、特性和潜在重氮营养活性

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Amanda C. Semler, Emily R. Paris, Mikaela Salvador, Anne E. Dekas
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引用次数: 0

摘要

重氮营养微生物利用部分由nifH基因编码的氮酶缓解海洋冷渗漏中的氮限制。在这里,我们利用扩增子测序和实时荧光定量PCR (qPCR)对nifH基因和转录本进行了研究,研究了6个生物地球化学异质性渗漏体内和体外含nifH的生物(NCOs)。我们检测到nifH基因与18个细菌和古菌门(17个在渗漏中)的系统发育和代谢多样性生物有关。检测到的nco包括甲烷氧化的ANME-2古菌和硫酸盐还原的desulobacteraceae,它们先前已被证明可以在渗漏处固定氮,以及Desulfuromonadales和假定的碳氢化合物氧化的Desulfoglaeba和Candidatus Methanoliparia。我们在6次渗漏中的5次中检测到nifH转录本,表明重氮营养活动广泛存在。我们根据扩增结果修正了qPCR数据,发现71%的恢复序列不是真正的nifH,我们建议在未来使用广泛的nifH引物的qPCR研究中进行类似的修正。NifH丰度在渗漏中高达3个数量级,与mcrA丰度相关,修正后与孔隙水铵(25 μM)负相关,与铵对重氮化的抑制作用一致。我们的发现扩大了渗漏处已知的nco的多样性,并强调了渗漏是深海重氮化的热点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Abundance, Identity, and Potential Diazotrophic Activity of nifH-Containing Organisms at Marine Cold Seeps

Abundance, Identity, and Potential Diazotrophic Activity of nifH-Containing Organisms at Marine Cold Seeps

Diazotrophic microorganisms alleviate nitrogen limitation at marine cold seeps using nitrogenase, encoded in part by the gene nifH. Here, we investigated nifH-containing organisms (NCOs) inside and outside six biogeochemically heterogeneous seeps using amplicon sequencing and quantitative real-time PCR (qPCR) of nifH genes and transcripts. We detected nifH genes affiliated with phylogenetically and metabolically diverse organisms spanning 18 bacterial and archaeal phyla (17 within seeps). Detected NCOs included methane-oxidising ANME-2 archaea and sulfate-reducing Desulfobacteraceae, which have been shown to fix nitrogen at seeps previously, as well as Desulfuromonadales and putatively hydrocarbon-oxidising Desulfoglaeba and Candidatus Methanoliparia. We detected nifH transcripts at five of the six seeps, suggesting widespread diazotrophic activity. We corrected our qPCR data based on our amplicon results, which found that 71% of recovered sequences were not bona fide nifH, and we recommend a similar correction in future qPCR studies that use broad nifH primers. NifH abundance was up to three orders of magnitude higher within seeps, was correlated with mcrA abundance, and, when corrected, was negatively correlated with porewater ammonium < 25 μM, consistent with the inhibition of diazotrophy by ammonium. Our findings expand the known diversity of NCOs at seeps and emphasise seeps as hotspots for deep-sea diazotrophy.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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