禽流感H5N1病毒2.3.4.4b进化支新重组的证据，印度，2024

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2025-03-05 DOI:10.1016/j.virol.2025.110482

Shailesh D. Pawar , Sachin S. Keng , Deeksha S. Tare , Anukumar Balakrishnan , Siba S , Jijo Koshy , Varsha Potdar , Veena Vipat , Satish Gaikwad , Dinesh Singh , Jayati Mullick , Naveen Kumar

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引用次数: 0

摘要

属于2.3.4.4b进化枝的H5N1病毒在全球引起了前所未有的暴发。据报告，2024年在喀拉拉邦（印度）的家禽和野鸟中暴发了H5N1病毒。从鸟类和环境中采集样本。采用实时荧光定量pcr和鸡胚分离病毒。20份样本中有8份病毒分离阳性。唾液酸酶测定显示该病毒具有禽型受体特异性。全基因组测序揭示了与高致病性和哺乳动物适应性相关的标记。所有病毒均属于具有多重重组的单一基因型，包括来自喀拉拉邦报告的禽流感（AI） H3N8病毒的内部基因片段。对可能接触者的监测显示没有人间感染。这是关于印度2.3.4.4b进化支H5N1病毒的遗传和病毒学特征的第一份报告，突出表明需要在人-动物和家鸟-野鸟界面加强人工智能监测。

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Evidence of novel reassortment in clade 2.3.4.4b avian influenza H5N1 viruses, India, 2024

H5N1 viruses belonging to clade 2.3.4.4b have caused unprecedented outbreaks globally. Outbreaks of H5N1 virus were reported in poultry and wild birds from Kerala (India) in the year 2024. Samples from birds and the environment were collected. Real-time RT-PCR and virus isolation using embryonated chicken eggs were carried out. Eight out of 20 samples were positive for virus isolation. The virus showed avian type receptor specificity using sialidase assay. Full genome sequencing revealed markers associated with high pathogenicity and mammalian adaptation. All the viruses belonged to a single genotype with multiple reassortments, including internal gene segments from an avian influenza (AI) H3N8 virus reported from Kerala. Surveillance among individuals with probable exposure showed absence of human infection. This is the first report of the genetic and virological characterization of clade 2.3.4.4b H5N1 viruses from India, highlighting the need for increased AI surveillance at the human-animal and domestic-wild bird interfaces.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.