肌腱工程共培养系统的现状与未来展望

Q3 Biochemistry, Genetics and Molecular Biology
Salomé Guillaumin , Andrea Rossoni , Dimitrios Zeugolis
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引用次数: 0

摘要

肌腱是连接骨骼和肌肉的结缔组织,可以维持骨骼姿势、关节运动、能量储存和肌肉力量向骨骼的传递。肌腱是一种自我再生能力较差的低细胞、低血管组织。目前的手术治疗效果有限,经常导致再损伤。即将到来的细胞疗法主要是基于腱细胞,一种体外自我更新能力有限的细胞群或间充质基质细胞,一种体内容易异位骨形成的细胞群。多年来,单因子或多因子细胞培养技术未能有效地在培养过程中维持细胞表型,也未能在植入前将间充质基质细胞培养成致腱细胞谱系。基于这些限制,共同文化的概念被构想出来了。在这里,我们全面回顾和讨论了间充质间质细胞通过直接或间接的肌腱细胞培养的成肌腱分化,试图产生用于再生医学目的的肌腱细胞或肌腱样细胞群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
State-of the-art and future perspective in co-culture systems for tendon engineering
Tendon is a connective tissue that links bone to muscle, allowing for maintenance of skeleton posture, joint movement, energy storage and transmission of muscle force to bone. Tendon is a hypocellular and hypovascular tissue of poor self-regeneration capacity. Current surgical treatments are of limited success, frequently resulting in reinjury. Upcoming cell therapies are primarily based on tenocytes, a cell population of limited self-renewal capacity in vitro or mesenchymal stromal cells, a cell population prone to ectopic bone formation in vivo. Over the years mono- or multi- factorial cell culture technologies have failed to effectively maintain tenocyte phenotype in culture during expansion or to prime mesenchymal stromal cells towards tenogenic lineage prior to implantation. Upon these limitations the concept of co-culture was conceived. Here, we comprehensively review and discuss tenogenic differentiation of mesenchymal stromal cells through direct or indirect culture with tenocytes in an attempt to generate a tenocyte or a tendon-like cell population for regenerative medicine purposes.
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来源期刊
CiteScore
4.10
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0.00%
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