利用先进的荧光生物传感技术照亮细胞外囊泡

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-03-08 DOI:10.1016/j.trac.2025.118228

Yeonju Lee , Kyung-Min Kim , Joonseok Lee , Young-Pil Kim

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引用次数: 0

摘要

细胞外囊泡（EVs）是细胞间通讯和重要生物过程的神秘信使，包括免疫调节、组织再生和疾病进展。尽管其在临床诊断方面具有巨大的价值，但由于其体积小且异质性，对ev的分析面临着重大挑战。本文综述了荧光生物传感技术的快速发展。FL生物传感技术与先进的生物技术和纳米技术的融合，已经详细阐述了EV分析，提供前所未有的灵敏度、特异性和多路复用能力。本文概述了电动汽车FL分析的三个关键领域：FL信号放大技术、Förster共振能量转移技术和创新FL技术。这些尖端的方法，利用荧光染料，能够精确地可视化和同时分析多个ev，提供对其不同亚群和生物过程潜在影响的见解，为新的诊断和治疗策略铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Illuminating extracellular vesicles with advanced fluorescence biosensing technologies

Extracellular vesicles (EVs) are the enigmatic messengers of intercellular communications and vital biological processes, including immune regulation, tissue regeneration, and disease progression. Despite their immense values as clinical diagnosis, the analysis of EVs poses significant challenges due to their small size and heterogeneity. This review focuses on the rapid evolution of fluorescence (FL) biosensing technologies. The convergence of FL biosensing technologies with advancements in biotechnology and nanotechnology has elaborated EV analysis, providing unprecedented sensitivity, specificity, and multiplexing capabilities. Here we overview three key areas of FL analyses of EVs: FL signal amplification technologies, Förster resonance energy transfer-based technologies, and innovative FL technologies. These cutting-edge methods, utilizing fluorescent dyes, enable precise visualization and simultaneous analysis of multiple EVs, offering insights into their diverse subpopulations and potential implications for biological processes, paving the way for novel diagnostic and therapeutic strategies.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.