伴侣动物作为危险的极重要抗微生物大肠杆菌的来源：上海基因组监测

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-03-10 DOI:10.1016/j.jhazmat.2025.137852

Yiwen Chen , Min Li , Bingqing Sun , Luming Xia , Yifei Zhang , Jun Feng , Min Chen , Zile Cheng , Chao Lv , Weiye Chen , Jiewen Huang , Qingtian Li , Yu Zhang , Jun Shang , Ying Cao , Tianyu Wang , Yanqi Liu , Mengmeng Wang , Bin Zhang , Xiaokui Guo , Yongzhang Zhu

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引用次数: 0

摘要

本研究首次对伴侣动物中至关重要的抗微生物（CIA）耐药性大肠杆菌进行了全面的基因组监测，解决了对城市生态系统中抗菌素耐药性（AMR）传播动态和危险抗性基因环境传播的理解空白。从上海11家宠物医院收集的730份肛门拭子中共分离出244株cia耐药大肠杆菌（CIA-EC）。高风险的人畜共患克隆，如ST410和ST405，在多个地区被发现，blandm -5阳性的IncX3质粒表现出卓越的稳定性、高效的结合和广泛的宿主适应性。这些质粒显著增加了blaNDM-5跨物种传播的全球风险。移动遗传元件（ISKox3, IS5, ISAba125）进一步促进了blaNDM-5的转移，增强了这些人畜共患病原体的持久性和适应性。此外，新的序列类型（ST87、ST12624）对包括替加环素（tet(X4)）在内的最后抗微生物药物表现出耐药性，从而加剧了公共卫生风险。研究结果表明，伴侣动物是人-动物-环境界面的关键宿主，促进了blandm -5阳性IncX3质粒等有害抗性元素的环境传播。将伴侣动物纳入“同一个健康”抗菌素耐药性监测和实施抗菌素管理，对于减轻cia耐药决定因素的传播和保持最后手段抗菌素的功效至关重要。

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Companion animals as sources of hazardous critically important antimicrobial-resistant Escherichia coli: Genomic surveillance in Shanghai

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Companion animals as sources of hazardous critically important antimicrobial-resistant Escherichia coli: Genomic surveillance in Shanghai

This study provides the first comprehensive genomic surveillance of critically important antimicrobial (CIA)-resistant Escherichia coli in companion animals, addressing gaps in the understanding of antimicrobial resistance (AMR) transmission dynamics and the environmental dissemination of hazardous resistance genes within urban ecosystems. A total of 244 CIA-resistant Escherichia coli (CIA-EC) isolates were obtained from 730 anal swabs collected across 11 pet hospitals in Shanghai. High-risk zoonotic clones, such as ST410 and ST405, were identified across multiple districts, with bla_NDM-5-positive IncX3 plasmids demonstrating exceptional stability, efficient conjugation, and broad host adaptability. These plasmids significantly amplify the global risk of cross-species bla_NDM-5 dissemination. Mobile genetic elements (ISKox3, IS5, ISAba125) further facilitated bla_NDM-5 transfer, enhancing the persistence and adaptability of these zoonotic pathogens. Additionally, novel sequence types (ST87, ST12624) exhibited resistance to last-resort antimicrobials, including tigecycline (tet(X4)), exacerbating the public health risks. The findings demonstrate that companion animals serve as critical reservoirs at the human-animal-environment interface, facilitating the environmental spread of hazardous resistance elements such as bla_NDM-5-positive IncX3 plasmids. Integrating companion animals into One Health AMR surveillance and implementing antimicrobial stewardship are crucial to mitigating the spread of CIA-resistance determinants and preserving the efficacy of last-resort antimicrobials.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.