聚对苯二甲酸乙二醇酯（PET）微塑料和PET渗滤液促进底栖蓝藻生长的分子机制

IF 12.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-03-10 DOI:10.1016/j.watres.2025.123476

Aonan Yang , Haiyan Pei , Ming Zhang , Yan Jin , Hangzhou Xu

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引用次数: 0

摘要

底栖蓝藻毒华严重威胁着淡水生态健康和饮用水安全。同时，微塑料污染日益严重，微塑料大量堆积在湖底河底，与藻类广泛共存。然而，微塑料对底栖蓝藻的影响尚不清楚。本研究研究了常见于湖底和河底的微塑料聚对苯二甲酸乙二醇酯（PET）及其环境相关浓度（0.3 mg/L）和高暴露浓度（3.0 mg/L）的渗滤液对典型底栖蓝藻（振荡藻sp.和假藻sp.）的影响，并通过转录组学分析阐明了相关分子机制。结果表明，PET或PET渗滤液（PET- l）均能促进底栖蓝藻的生长，且PET- l的促进作用比PET体系更明显。PET或PET- l在环境相关浓度下的促进作用比高暴露浓度下的促进作用更显著（比对照组增加39-63%），比对照组增加21-58%。在PET或PET- l存在的情况下，由于蓝藻细胞数量的增加，水中有害代谢物（柱体精子素、土臭素和2-甲基异龙脑）的浓度也增加了。尽管PET颗粒可能由于遮荫效应和机械损伤而不利于底栖蓝藻的生长，但转录组分析表明，PET颗粒提高了藻类的光合效率，上调了与光合作用和糖脂细胞外转运相关的异常基因。此外，PET分解组分，如对苯二甲酸和乙二醇，可能能够作为蓝藻生长的碳源。糖酵解、氧化磷酸化和翻译相关基因的上调表明，PET可以促进底栖蓝藻的生长。本研究对评价微塑料共存条件下底栖蓝藻对水生生态健康和饮用水安全的影响具有重要价值。

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Molecular mechanisms by which polyethylene terephthalate (PET) microplastic and PET leachate promote the growth of benthic cyanobacteria

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Molecular mechanisms by which polyethylene terephthalate (PET) microplastic and PET leachate promote the growth of benthic cyanobacteria

Toxic blooms of benthic cyanobacteria greatly threaten freshwater ecological health and drinking water safety. Meanwhile, microplastic pollution is becoming increasingly severe and microplastics accumulate in large quantities at the bottom of lakes and rivers, widely coexisting with algae. However, impacts of microplastics on benthic cyanobacteria are still unknown. This study investigated effects of microplastic polyethylene terephthalate (PET) — which is commonly found at the bottom of lakes and rivers — and its leachate at environmentally relevant concentration (0.3 mg/L) and high exposure concentration (3.0 mg/L) on typical benthic cyanobacteria (Oscillatoria sp. and Pseudanabaena sp.), and clarified the related molecular mechanisms through transcriptomic analysis. Results show that PET or PET leachate (PET-L) can promote benthic cyanobacterial growth and promotive effect of PET-L is more obvious than that of PET system. Promotion effect of PET or PET-L is more significant at environmentally relevant concentration (39–63 % increase compared with the control) compared with high exposure concentration (21–58 % increase compared with the control). In the presence of PET or PET-L, due to an increase in the number of cyanobacterial cells, concentrations of harmful metabolites (cylindrospermopsin, geosmin, and 2-methylisoborneol) in water also increased. Although PET particles may not be conducive to benthic cyanobacterial growth due to shading effect and mechanical damage, photosynthetic efficiency of algae was improved and dysregulated genes related to photosynthesis and extracellular transport of glycolipid were upregulated according to transcriptome analysis. Moreover, PET decomposition components, such as terephthalic acid and ethylene glycol, may be able to serve as carbon sources for cyanobacterial growth. Upregulation of genes associated with glycolysis, oxidative phosphorylation, and translation revealed that PET can promote the growth of benthic cyanobacteria. This study has important value in evaluating the impact of benthic cyanobacteria on aquatic ecological health and drinking water safety with the coexistence of microplastics.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.