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IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-03-10 DOI:10.1016/j.envpol.2025.126039

Hongsong Chen , Hao Chen , Lian Hua , Yunqi Gao , Yuehua Yao , Yangfan Xu , Jingfeng Yang , Lingtian Xie , Yongju Luo , Ting Huang , Hongxing Chen , Xuguang Lin , Wu Dong

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引用次数: 0

摘要

全球变暖会导致二恶英类沉积物的释放和地理迁移的增加，从而增加人类和动物接触二恶英的风险。本实验以CYP1A转基因斑马鱼Tg（cyp1a：mCherry）和肝脏荧光转基因斑马鱼Tg（fabp10：Ps Red）为动物模型，分别在26℃和30℃条件下暴露于2,3,7,8-四氯二苯并对二恶英（TCDD）。研究人员检测了这些动物的形态学变化、组织学变化、转录组和相关基因的表达。结果表明，与 26 ℃ 相比，暴露于 30 ℃ 的 TCDD 会增加斑马鱼幼体的死亡率和心包腔面积，并减少肝细胞数量。转录组分析表明，TCDD 显著改变了过氧化物酶体增殖激活受体（PPARs）代谢途径、脂肪细胞因子、脂肪酸降解和细胞死亡。qRT-PCR还检测到，与26 °C水温孵育相比，在30 °C下暴露于TCDD诱导的斑马鱼幼体中，ahr2、cyp相关基因（cyp1.1、cyp1b1、cyp1c1和cyp3a65）和PPARs（pparα、pparβ和pparγ）的表达进一步显著增加。我们的结果表明，环境温度升高（从 26 °C 升至 30 °C）导致 TCDD 诱导的肝毒性更加强烈。观察到的毒性变化可能与脂质过氧化有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effects of combined stressors to TCDD and high temperature on HSP/CYPs signaling in the zebrafish embryos/larvae

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Effects of combined stressors to TCDD and high temperature on HSP/CYPs signaling in the zebrafish embryos/larvae

Global warming causes the release of dioxin-like deposits and increases geographical migration, increasing the risk of exposure for humans and animals. In this experiment, we used CYP1A transgenic zebrafish Tg (cyp1a: mCherry) and liver fluorescent transgenic zebrafish Tg (fabp10: Ps Red) as an animal model and exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) at 26 °C and 30 °C, respectively. Morphological changes, histological changes, transcriptome and related genes expression were detected. The results showed that exposure to TCDD at 30 °C increased the mortality rate, pericardial cavity area, and reduced the number of liver cells in zebrafish larvae compared to 26 °C. Transcriptome analysis showed that, TCDD significantly altered Peroxisome Proliferators-Activated Receptors (PPARs) metabolic pathway, adipocytokine, fatty acid degradation and cell death. qRT-PCR also detected a further significant increase in the expression of ahr2, cyp-related genes (cyp1.1, cyp1b1, cyp1c1 and cyp3a65), and PPARs (pparα, pparβ and pparγ) in zebrafish larvae induced by TCDD exposure under 30 °C compared to 26 °C water temperature incubation. Our results showed that the increase of ambient temperature (from 26 °C to 30 °C) causes TCDD induced hepatotoxicity to be more intense. The observed toxic changes were likely related to lipid peroxidation.

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.