Hongsong Chen , Hao Chen , Lian Hua , Yunqi Gao , Yuehua Yao , Yangfan Xu , Jingfeng Yang , Lingtian Xie , Yongju Luo , Ting Huang , Hongxing Chen , Xuguang Lin , Wu Dong
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Effects of combined stressors to TCDD and high temperature on HSP/CYPs signaling in the zebrafish embryos/larvae
Global warming causes the release of dioxin-like deposits and increases geographical migration, increasing the risk of exposure for humans and animals. In this experiment, we used CYP1A transgenic zebrafish Tg (cyp1a: mCherry) and liver fluorescent transgenic zebrafish Tg (fabp10: Ps Red) as an animal model and exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) at 26 °C and 30 °C, respectively. Morphological changes, histological changes, transcriptome and related genes expression were detected. The results showed that exposure to TCDD at 30 °C increased the mortality rate, pericardial cavity area, and reduced the number of liver cells in zebrafish larvae compared to 26 °C. Transcriptome analysis showed that, TCDD significantly altered Peroxisome Proliferators-Activated Receptors (PPARs) metabolic pathway, adipocytokine, fatty acid degradation and cell death. qRT-PCR also detected a further significant increase in the expression of ahr2, cyp-related genes (cyp1.1, cyp1b1, cyp1c1 and cyp3a65), and PPARs (pparα, pparβ and pparγ) in zebrafish larvae induced by TCDD exposure under 30 °C compared to 26 °C water temperature incubation. Our results showed that the increase of ambient temperature (from 26 °C to 30 °C) causes TCDD induced hepatotoxicity to be more intense. The observed toxic changes were likely related to lipid peroxidation.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.