不同荚膜血清型高毒力和耐碳青霉烯肺炎克雷伯菌的流行病学、表型和基因型特征差异

IF 3.7 2区医学 Q2 IMMUNOLOGY

Journal of Microbiology Immunology and Infection Pub Date : 2025-02-28 DOI:10.1016/j.jmii.2025.02.010

Cong Zhou , Wencai Ke , Hui Zhang , Maosuo Xu , Baoyu Yuan , Yong Lin , Fang Shen

{"title":"不同荚膜血清型高毒力和耐碳青霉烯肺炎克雷伯菌的流行病学、表型和基因型特征差异","authors":"Cong Zhou , Wencai Ke , Hui Zhang , Maosuo Xu , Baoyu Yuan , Yong Lin , Fang Shen","doi":"10.1016/j.jmii.2025.02.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance and hypervirulence phenotypes, but the differences in epidemiological, phenotypic and genotypic characteristics between them remains unclear.</div></div><div><h3>Methods</h3><div>In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates in a tertiary hospital in Shanghai, China from January 2019 to December 2022. Furthermore, we selected four typical hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em>, including ST23-KL1/ST86-KL2 CR-hvKP (WYKP3 and WYKP194) and ST11-KL64/ST11-KL47 hv-CRKP (WYKP589 and WYKP188), and tried to clarify and compare their differences in virulence and drug resistance characteristics and plasmid distribution.</div></div><div><h3>Results</h3><div>Our study found that ST23-KL1 and ST86-KL2 CR-hvKP exhibited less plasmid diversity than that of ST11-KL64 and ST11-KL47 hv-CRKP. Compared with ST11-KL64 and ST11-KL47 hv-CRKP, ST23-KL1/ST86-KL2 CR-hvKP harbored significantly fewer antimicrobial resistance genes but more virulence genes, which contributed to the higher virulence of these strains and exhibited resistance to fewer antibiotics. ST11-KL64 hv-CRKP has emerged as the most prevalent hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em> probably due to its clonal transmission within hospitals as well as the transmission of virulence plasmids with the help of conjugative resistance plasmids.</div></div><div><h3>Conclusions</h3><div>Due to the different evolutionary mechanisms of hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em> with different capsular serotypes, the epidemiological, phenotypic and genotypic characteristics of KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP are different.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"58 4","pages":"Pages 444-454"},"PeriodicalIF":3.7000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Epidemiological, phenotypic and genotypic characteristics difference of hypervirulent and carbapenem-resistant Klebsiella pneumoniae with different capsular serotypes\",\"authors\":\"Cong Zhou , Wencai Ke , Hui Zhang , Maosuo Xu , Baoyu Yuan , Yong Lin , Fang Shen\",\"doi\":\"10.1016/j.jmii.2025.02.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance and hypervirulence phenotypes, but the differences in epidemiological, phenotypic and genotypic characteristics between them remains unclear.</div></div><div><h3>Methods</h3><div>In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates in a tertiary hospital in Shanghai, China from January 2019 to December 2022. Furthermore, we selected four typical hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em>, including ST23-KL1/ST86-KL2 CR-hvKP (WYKP3 and WYKP194) and ST11-KL64/ST11-KL47 hv-CRKP (WYKP589 and WYKP188), and tried to clarify and compare their differences in virulence and drug resistance characteristics and plasmid distribution.</div></div><div><h3>Results</h3><div>Our study found that ST23-KL1 and ST86-KL2 CR-hvKP exhibited less plasmid diversity than that of ST11-KL64 and ST11-KL47 hv-CRKP. Compared with ST11-KL64 and ST11-KL47 hv-CRKP, ST23-KL1/ST86-KL2 CR-hvKP harbored significantly fewer antimicrobial resistance genes but more virulence genes, which contributed to the higher virulence of these strains and exhibited resistance to fewer antibiotics. ST11-KL64 hv-CRKP has emerged as the most prevalent hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em> probably due to its clonal transmission within hospitals as well as the transmission of virulence plasmids with the help of conjugative resistance plasmids.</div></div><div><h3>Conclusions</h3><div>Due to the different evolutionary mechanisms of hypervirulent and carbapenem-resistant <em>Klebsiella pneumoniae</em> with different capsular serotypes, the epidemiological, phenotypic and genotypic characteristics of KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP are different.</div></div>\",\"PeriodicalId\":56117,\"journal\":{\"name\":\"Journal of Microbiology Immunology and Infection\",\"volume\":\"58 4\",\"pages\":\"Pages 444-454\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microbiology Immunology and Infection\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S168411822500043X\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microbiology Immunology and Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S168411822500043X","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景：KL1、KL2 CR-hvKP和KL64、KL47 hv-CRKP均表现出重叠的多药耐药和高毒力表型，但它们之间在流行病学、表型和基因型特征上的差异尚不清楚。方法：在本研究中，我们收集了2019年1月至2022年12月在中国上海某三级医院非重复的hv-CRKP/CR-hvKP分离株。此外，我们选取了ST23-KL1/ST86-KL2 CR-hvKP （WYKP3和WYKP194）和ST11-KL64/ST11-KL47 hv-CRKP (WYKP589和WYKP188) 4种典型的高毒性和耐碳青霉烯类肺炎克雷伯菌，试图阐明和比较它们在毒力、耐药特性和质粒分布上的差异。结果：我们的研究发现ST23-KL1和ST86-KL2 CR-hvKP的质粒多样性低于ST11-KL64和ST11-KL47 hv-CRKP。与ST11-KL64和ST11-KL47 hv-CRKP相比，ST23-KL1/ST86-KL2 CR-hvKP携带的耐药基因明显少于ST11-KL64和ST11-KL47 hv-CRKP，但携带的毒力基因较多，这导致这些菌株的毒力更高，对更少的抗生素产生耐药性。ST11-KL64 hv-CRKP已成为最普遍的高致病性和耐碳青霉烯肺炎克雷伯菌，这可能是由于其在医院内的克隆传播以及在偶联耐药质粒的帮助下毒力质粒的传播。结论：由于不同荚膜血清型的高毒力和耐碳青霉烯肺炎克雷伯菌的进化机制不同，导致KL1、KL2 CR-hvKP和KL64、KL47 hv-CRKP的流行病学、表型和基因型特征不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Epidemiological, phenotypic and genotypic characteristics difference of hypervirulent and carbapenem-resistant Klebsiella pneumoniae with different capsular serotypes

Background

KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance and hypervirulence phenotypes, but the differences in epidemiological, phenotypic and genotypic characteristics between them remains unclear.

Methods

In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates in a tertiary hospital in Shanghai, China from January 2019 to December 2022. Furthermore, we selected four typical hypervirulent and carbapenem-resistant Klebsiella pneumoniae, including ST23-KL1/ST86-KL2 CR-hvKP (WYKP3 and WYKP194) and ST11-KL64/ST11-KL47 hv-CRKP (WYKP589 and WYKP188), and tried to clarify and compare their differences in virulence and drug resistance characteristics and plasmid distribution.

Results

Our study found that ST23-KL1 and ST86-KL2 CR-hvKP exhibited less plasmid diversity than that of ST11-KL64 and ST11-KL47 hv-CRKP. Compared with ST11-KL64 and ST11-KL47 hv-CRKP, ST23-KL1/ST86-KL2 CR-hvKP harbored significantly fewer antimicrobial resistance genes but more virulence genes, which contributed to the higher virulence of these strains and exhibited resistance to fewer antibiotics. ST11-KL64 hv-CRKP has emerged as the most prevalent hypervirulent and carbapenem-resistant Klebsiella pneumoniae probably due to its clonal transmission within hospitals as well as the transmission of virulence plasmids with the help of conjugative resistance plasmids.

Conclusions

Due to the different evolutionary mechanisms of hypervirulent and carbapenem-resistant Klebsiella pneumoniae with different capsular serotypes, the epidemiological, phenotypic and genotypic characteristics of KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP are different.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES

CiteScore

15.90

自引率

5.40%

发文量

159

审稿时长

67 days

期刊介绍： Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.