维生素E和胆固醇一样吗?用和频振动光谱法研究维生素E对磷脂膜结构和动力学的影响。

IF 3.2 3区 生物学 Q2 BIOPHYSICS
Joshua M Taylor, Kai H Gerton, John C Conboy
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引用次数: 0

摘要

维生素E (VE)历来被认为是一种抗氧化剂,其在清除自由基和营养方面的作用也得到了很好的研究。VE被认为在膜内具有次要作用,但这些作用并没有很好地表征,在整个文献中出现了相互矛盾的结果。由于相似的结构基序,VE和另一种膜成分胆固醇(CHO)之间的比较已被普遍采用。尽管这些比较表明磷脂-CHO和磷脂-VE相互作用的行为可能相似,但与CHO的影响相比,VE对磷脂翻转的潜在影响还没有得到很好的研究。在这里,我们通过使用和频率振动光谱显示,在生物(0.5-1.5 mol. %)和超生理(2.5-5 mol. %)浓度下,VE在饱和脂质1,2-双棕榈酰- n-甘油-3-磷脂胆碱(DPPC)的平面支撑脂质双层(pslb)内诱导磷脂的烷基链有序的能力与CHO相似。除了链排序外,VE的引入使磷脂的翻转加速了大约三倍(0.5-2.5 mol. %),在高VE含量下,速率接近数量级增加(5 mol. %)。磷脂翻转率的增加归因于膜的摩尔压缩模量的降低。这些结果表明,VE对膜的有序性和可压缩性的影响与CHO相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Does vitamin E behave like cholesterol? An examination of vitamin E's effects on phospholipid membrane structure and dynamics through sum-frequency vibrational spectroscopy.

Vitamin E (VE) has historically been described as an antioxidant and its roles in radical species scavenging and nutrition are well studied. VE has been proposed to have secondary roles within the membrane but these roles are not as well characterized, with contradictory results emerging throughout the literature. Due to similar structural motifs, comparisons between VE and cholesterol (CHO), another membrane component, have been commonly made. Despite these comparisons showing that phospholipid-CHO and phospholipid-VE interactions may behave similarly, VE's potential influence on phospholipid flip-flop specifically is not as well studied when compared with CHO's influence. Here, we show through the use of sum-frequency vibrational spectroscopy that VE at both biological (0.5-1.5 mol %) and supraphysiological (2.5-5 mol %) concentrations shows similar characteristics to that of CHO in its ability to induce alkyl chain ordering of phospholipids within planar supported lipid bilayers of the saturated lipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. In addition to chain ordering, the introduction of VE accelerates phospholipid flip-flop by approximately three times (0.5-2.5 mol %) with rates approaching an order-of-magnitude increase (5 mol %) at high VE content. The increase in phospholipid flip-flop rates is attributed to the decrease in the molar compression modulus of the membrane. These results suggest that VE influences the ordering and compressibility of the membrane similar to CHO.

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来源期刊
Biophysical journal
Biophysical journal 生物-生物物理
CiteScore
6.10
自引率
5.90%
发文量
3090
审稿时长
2 months
期刊介绍: BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.
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