挤奶间充质干细胞：细胞裂解液、分泌组和外泌体提取的更新方案，以及它们治疗潜力的比较分析。

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2025-03-07 DOI:10.1016/j.ymeth.2025.03.004

Sepideh Zununi Vahed , Seyyedeh Mina Hejazian , William Ndjidda Bakari , Rebecca Landon , Virginie Gueguen , Anne Meddahi-Pellé , Fani Anagnostou , Abolfazl Barzegari , Graciela Pavon-Djavid

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引用次数: 0

摘要

间充质干细胞（MSCs）的细胞裂解物、分泌组和细胞外囊泡（ev）调节免疫反应和促进组织再生的潜力使它们成为无细胞治疗的一个有前途的选择。目前，许多干细胞衍生的ev和分泌组的临床试验正在进行中，用于治疗各种各样的疾病，有时结果是失败的。该路线图的主要挑战是缺乏外泌体、分泌组和裂解物的标准提取方法。获得临床使用的MSCs分泌组的最佳方法包括非破坏性收集方法、时间优化、多轮收集、培养条件优化和质量控制措施。需要进一步的研究和临床研究来验证和完善这些方法，以便在各种临床应用中安全有效地利用MSC外泌体、分泌组和裂解物。为了应对这些挑战，必须建立一个标准化和统一的方法，以确保在临床试验中对这些提取物进行可靠的评估。本文综述了mscs衍生的外泌体和分泌组/裂解物制备方法的优缺点，并对其治疗潜力进行了比较分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Milking mesenchymal stem cells: Updated protocols for cell lysate, secretome, and exosome extraction, and comparative analysis of their therapeutic potential

The potential of the cell lysate, secretome, and extracellular vesicles (EVs) of mesenchymal stem cells (MSCs) to modulate the immune response and promote tissue regeneration has positioned them as a promising option for cell-free therapy. Currently, many clinical trials in stem cells-derived EVs and secretome are in progress various diseases and sometimes the results are failing. The major challenge on this roadmap is the lack of a standard extraction method for exosome, secretome, and lysate. The most optimal method for obtaining the secretome of MSCs for clinical utilization involves a comprehensive approach that includes non-destructive collection methods, time optimization, multiple collection rounds, optimization of culture conditions, and quality control measures. Further research and clinical studies are warranted to validate and refine these methods for safe and effective utilization of the MSC exosome, secretome, and lysate in various clinical applications. To address these challenges, it is imperative to establish a standardized and unified methodology to ensure reliable evaluation of these extractions in clinical trials. This review seeks to outline the pros and cons of methods for the preparation of MSCs-derived exosome, and secretome/lysate, and comparative analysis of their therapeutic potential.

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.