{"title":"基于喹啉的荧光探针定量检测婴儿配方奶粉和活细胞中的Zn2+","authors":"Wen Lu, Jichao Chen, Jiongya Tang, Yutian chen, Yingying Ma, Wuhan Sang, Sicheng Feng, Shilong Yang, Yanqin Wang, Xu Li","doi":"10.1007/s12161-024-02709-3","DOIUrl":null,"url":null,"abstract":"<div><p>Zinc is an essential trace element and its deficiency has been related to skin conditions, Alzheimer’s disease, and some types of cancer. Therefore, detecting zinc ions in the human body with high sensitivity is important. Here, two “turn-on” quinoline-based fluorescent probes (<i>E</i>)-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (<b>QSP-H</b>) and (<i>E</i>)-4-chloro-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (<b>QSP-Cl</b>) were fabricated for the detection of Zn<sup>2+</sup>. Both the <b>QSP-H</b> and <b>QSP-Cl</b> revealed low LOD (71 nM for <b>QSP-H</b>, 67 nM for <b>QSP-Cl</b>) and high selectivity, and worked across a broad pH range (3 ‒12 for <b>QSP-H</b>, 3 ‒11 for <b>QSP-Cl</b>). The HRMS, <sup>1</sup>H NMR titration, DFT calculations and Job’s plot analysis were employed to explore the mechanism of Zn<sup>2+</sup> detection through <b>QSP-H</b> and <b>QSP-Cl</b>. <b>QSP-H</b> and <b>QSP-Cl</b> were effectively applied to the quantitative assessment of Zn<sup>2+</sup> in two infant formula samples and to the bioimaging-based detection of exogenous Zn<sup>2+</sup> in living cells.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 2","pages":"190 - 201"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative Detection of Zn2+ in Infant Formula and Living Cells Using Quinoline-Based Fluorescent Probes\",\"authors\":\"Wen Lu, Jichao Chen, Jiongya Tang, Yutian chen, Yingying Ma, Wuhan Sang, Sicheng Feng, Shilong Yang, Yanqin Wang, Xu Li\",\"doi\":\"10.1007/s12161-024-02709-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Zinc is an essential trace element and its deficiency has been related to skin conditions, Alzheimer’s disease, and some types of cancer. Therefore, detecting zinc ions in the human body with high sensitivity is important. Here, two “turn-on” quinoline-based fluorescent probes (<i>E</i>)-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (<b>QSP-H</b>) and (<i>E</i>)-4-chloro-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (<b>QSP-Cl</b>) were fabricated for the detection of Zn<sup>2+</sup>. Both the <b>QSP-H</b> and <b>QSP-Cl</b> revealed low LOD (71 nM for <b>QSP-H</b>, 67 nM for <b>QSP-Cl</b>) and high selectivity, and worked across a broad pH range (3 ‒12 for <b>QSP-H</b>, 3 ‒11 for <b>QSP-Cl</b>). The HRMS, <sup>1</sup>H NMR titration, DFT calculations and Job’s plot analysis were employed to explore the mechanism of Zn<sup>2+</sup> detection through <b>QSP-H</b> and <b>QSP-Cl</b>. <b>QSP-H</b> and <b>QSP-Cl</b> were effectively applied to the quantitative assessment of Zn<sup>2+</sup> in two infant formula samples and to the bioimaging-based detection of exogenous Zn<sup>2+</sup> in living cells.</p></div>\",\"PeriodicalId\":561,\"journal\":{\"name\":\"Food Analytical Methods\",\"volume\":\"18 2\",\"pages\":\"190 - 201\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Analytical Methods\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s12161-024-02709-3\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02709-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Quantitative Detection of Zn2+ in Infant Formula and Living Cells Using Quinoline-Based Fluorescent Probes
Zinc is an essential trace element and its deficiency has been related to skin conditions, Alzheimer’s disease, and some types of cancer. Therefore, detecting zinc ions in the human body with high sensitivity is important. Here, two “turn-on” quinoline-based fluorescent probes (E)-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (QSP-H) and (E)-4-chloro-2-((2-(quinolin-2-yl) hydrazono) methyl) phenol (QSP-Cl) were fabricated for the detection of Zn2+. Both the QSP-H and QSP-Cl revealed low LOD (71 nM for QSP-H, 67 nM for QSP-Cl) and high selectivity, and worked across a broad pH range (3 ‒12 for QSP-H, 3 ‒11 for QSP-Cl). The HRMS, 1H NMR titration, DFT calculations and Job’s plot analysis were employed to explore the mechanism of Zn2+ detection through QSP-H and QSP-Cl. QSP-H and QSP-Cl were effectively applied to the quantitative assessment of Zn2+ in two infant formula samples and to the bioimaging-based detection of exogenous Zn2+ in living cells.
期刊介绍：
Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.
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