茶树F-box-LRR基因家族的全基因组鉴定及CsFBXL13转录因子的功能分析

IF 3.9 4区 生物学 Q1 GENETICS & HEREDITY
Xiangya Dou, Siyi Xie, Jinbo Wang, Xiaohua Shen, Shuoqian Liu, Na Tian
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引用次数: 0

摘要

本研究对茶树F-box-LRR (FBXL)蛋白家族进行了鉴定和功能分析,旨在揭示其在春芽萌发和环境适应中的作用。利用生物信息学方法,对茶树F-box-LRR基因家族的37个成员进行了染色体定位、基因结构、保守基序和顺式作用元件的系统分析。结果表明,这些基因分布在14条染色体上,具有较强的保守性和基因间协方差特征。顺式作用元件分析表明,F-box-LRR家族成员与低温、赤霉素和生长激素等信号相关,可能在春季低温萌发中起关键作用。此外,本研究通过亚细胞定位、酵母单杂交和双荧光素酶实验验证了CsWRKY40转录因子直接结合到CsFBXL13基因的启动子区域,并显著激活其表达,揭示了CsWRKY40-CsFBXL13调控轴在茶树低温响应和春芽萌发中的重要作用。本研究不仅拓展了对F-box-LRR蛋白家族的认识,也为通过分子育种提高茶树的抗性和生产力提供了潜在的分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide identification of F-box-LRR gene family and the functional analysis of CsFBXL13 transcription factor in tea plants

This study focused on the identification and functional analysis of the F-box-LRR (FBXL) protein family in tea tree (Camellia sinensis), aiming to reveal its role in spring bud germination and environmental adaptation. Thirty-seven members of the tea tree F-box-LRR gene family were identified and systematically analyzed for their chromosomal localization, gene structure, conserved motifs, and cis-acting elements by bioinformatics methods. It was found that these genes were distributed on 14 chromosomes, with strong conserved and inter-gene covariance characteristics. Cis-acting element analysis showed that the F-box-LRR family members were associated with signals such as low temperature, gibberellin and growth hormone, which may play a key role in spring low-temperature germination. In addition, the study verified that the CsWRKY40 transcription factor directly binds to the promoter region of the CsFBXL13 gene and significantly activates its expression by subcellular localization, yeast one-hybridization and dual luciferase assays, revealing the important function of the CsWRKY40-CsFBXL13 regulatory axis in low-temperature response and spring bud germination in tea tree. This study not only expands the understanding of the F-box-LRR protein family, but also provides potential molecular targets for improving the resistance and productivity of tea tree through molecular breeding.

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来源期刊
CiteScore
3.50
自引率
3.40%
发文量
92
审稿时长
2 months
期刊介绍: Functional & Integrative Genomics is devoted to large-scale studies of genomes and their functions, including systems analyses of biological processes. The journal will provide the research community an integrated platform where researchers can share, review and discuss their findings on important biological questions that will ultimately enable us to answer the fundamental question: How do genomes work?
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