嗜酸乳杆菌提取的碳点单独或混合使用对耐碳青霉烯类肺炎克雷伯菌的协同抗菌作用

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-02-28 DOI:10.1016/j.jiph.2025.102724

Leila Shamkhali , Ashraf Mohabati Mobarez , Seyed Davar Siadat , Hamid Pajavand

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L-C-dots were synthesized using a hydrothermal method and characterized. Furthermore, their antibacterial and anti-biofilm activities were evaluated against CRKP isolates. The combination of L-C-dots with the meropenem antibiotics were also tested using a checkerboard assay. Finally, the influence of L-C-dots on the expression levels of <em>fimH</em> and <em>mrkD</em> genes was examined using quantitative PCR (qPCR).</div></div><div><h3>Results</h3><div>L-C-dots demonstrated significant antibacterial activity against CRKP isolates, with a minimum inhibitory concentration (MIC) of 50 mg/mL and a sub-MIC of 25 mg/mL. L-C-dots effectively inhibited biofilm formation at MIC and sub-MIC concentrations (P ˂ 0.05). Additionally, the L-C-dots had synergistic and additive interactions with the meropenem antibiotics against CRKP isolates, reducing the MIC of both agents. 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Synergistic antibacterial effects of carbon dots derived from Lactobacillus acidophilus alone and in combination against carbapenem-resistant Klebsiella pneumoniae

Background

Non-antibiotic treatments, such as carbon dots (C-dots), are gaining popularity in the search for effective alternatives. We aimed to evaluate the antibacterial properties of C-dots derived from Lactobacillus acidophilus (L-C-dots) alone and in combination against carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates.

Methods

Seventy clinical isolates of Klebsiella pneumoniae were tested for carbapenem resistance using a modified carbapenem inactivation assay. To determine the presence of fimH and mrkD genes, which are associated with biofilm formation, polymerase chain reaction (PCR) was conducted. L-C-dots were synthesized using a hydrothermal method and characterized. Furthermore, their antibacterial and anti-biofilm activities were evaluated against CRKP isolates. The combination of L-C-dots with the meropenem antibiotics were also tested using a checkerboard assay. Finally, the influence of L-C-dots on the expression levels of fimH and mrkD genes was examined using quantitative PCR (qPCR).

Results

L-C-dots demonstrated significant antibacterial activity against CRKP isolates, with a minimum inhibitory concentration (MIC) of 50 mg/mL and a sub-MIC of 25 mg/mL. L-C-dots effectively inhibited biofilm formation at MIC and sub-MIC concentrations (P ˂ 0.05). Additionally, the L-C-dots had synergistic and additive interactions with the meropenem antibiotics against CRKP isolates, reducing the MIC of both agents. Furthermore, the L-C-dots decreased the expression of the fimH (p< 0.029) and mrkD (p < 0.015) genes.

Conclusions

The findings indicate that L-C-dots may serve as a promising new treatment option for CRKP infections. They show potential as a strong therapeutic choice, especially when used in combination with traditional antibiotics.

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.