阿根廷农业生态和工业系统中饲养家兔（Oryctolagus cuniculus）的艾美耳球虫

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-03-06 DOI:10.1016/j.vprsr.2025.101234

Kevin Denis Steffen , Marin Juan Carlos , Basset Carina , Trigo María Soledad , Arias Ruben Omar , Unzaga Juan Manuel , Moré Gastón

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引用次数: 0

摘要

兔球虫病是影响兔健康的主要因素。在本研究中，来自农业生态系统（n = 11）和工业系统（n = 79）的90只2.7-5月龄、1.5-2.5公斤的杂交兔子（新西兰x加利福尼亚）被屠宰食用。收集胆汁和肝脏样本，过滤，溶解在重铬酸钾和蒸馏水中进行卵囊产孢。此外，肝组织样本固定在10%的缓冲福尔马林分析组织病理学诊断。总阳性率为24.4%（22/90）。农业生态系统患病率为72.7%(8/11)，工业系统患病率为17.7%（14/79）。27.2%（6/22）的胆囊囊穿刺后，在内部可见非孢子性卵囊，与肝肿大的肝脏一致，肝表面和内部有较多的黄白色结节。肝轻度肿大，各肝叶可见大小不等的不规则黄白色结节，含白色干酪样物质及无孢子卵囊。胆囊肿胀（3.5 cm），伴有淡黄色液体。组织病理学分析显示胆管因广泛的上皮增生而扩张，有大量坏死内容物和脱落的胆管上皮细胞，同时在胆管腔内有厚壁的非孢子卵泡。序列分析表明，该引物与兔e.s tiedae 18S通用引物及已存入GenBank的序列（PQ427813）同源性为98.4%。对ITS1产物进行序列分析，结果表明该产物与家兔的同源性为92.2 ~ 93.4%。本文通过形态学、组织病理学和分子病理学的证实，描述了阿根廷农业生态和工业系统中兔肝脏的自然感染。我们的结果可能有助于阿根廷家兔感染伊氏杆菌的诊断。

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Eimeria stiedae in domestic rabbits (Oryctolagus cuniculus) farmed in agroecological and industrial systems in Argentina

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Eimeria stiedae in domestic rabbits (Oryctolagus cuniculus) farmed in agroecological and industrial systems in Argentina

Health in commercial and domestic rabbit farming is commonly affected by rabbit coccidiosis. For this study, 90 crossbreed rabbits (New Zealand x Californian) of 2.7–5 months of age and 1.5–2.5 kg from agroecological (n = 11) and industrial (n = 79) systems were slaughtered for consumption. Bile and liver samples were collected, filtered, and dissolved in potassium dichromate and distilled water for oocyst sporulation. Additionally, liver tissue samples fixed in 10 % buffered formalin were analysed for histopathological diagnosis. The overall prevalence of E. stiedae was observed in 24.4 % (22/90) of the liver samples. The prevalence in the agroecological system was 72.7 % (8/11) and 17.7 % (14/79) in the industrial system. In 27.2 % (6/22) bile vesicles, after a puncture, non-sporulating oocysts were observed in the interior, coinciding with those livers with hepatomegaly and a greater number of yellowish-white nodules, scattered for the surface and in the interior. Mild hepatomegaly was observed, with irregular yellowish-white nodules of varying sizes in all liver lobes, containing white caseous material with non-sporulating oocysts. The gall bladder was distended (3.5 cm) with yellowish fluid. Histopathological analysis showed dilated bile ducts due to extensive epithelial proliferation and hyperplasia, with abundant necrotic contents and detached biliary epithelial cells, alongside thick-walled non-sporulating oocysts in the duct lumen. Sequence analysis showed 98.4 % homology with rabbit E. stiedae 18S generic primers and the sequences deposited in GenBank such as (PQ427813). The sequence analysis obtained from the ITS1 product showed 92.2–93.4 % homology with rabbit E. stiedae. This report describes the natural infection of E. stiedae in rabbit liver from the agroecological and industrial system by morphology, histopathology, and molecular confirmation in Argentina. Our results could contribute to the diagnosis of E. stiedae infection in rabbits in Argentina.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).