一种用于饮用水生产的全尺寸慢沙过滤器的真菌群落中，罗氏菌门的未表征成员占主导地位。

IF 12.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-03-08 DOI:10.1016/j.watres.2025.123447

Tage Rosenqvist , Sandy Chan , Catherine J. Paul

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引用次数: 0

摘要

用于饮用水生产的慢沙过滤器（ssf）是来自多个生命领域的各种微生物的栖息地，这是ssf净化水能力不可或缺的一部分。虽然对ssf的培养独立的原核群落分析提供了有价值的见解，但很少关注寄生在ssf上的真菌。本研究对已建立、接种和未接种三种菌种的沙土生物膜中的真菌群落进行了研究。通过去除表层沙子（“刮”），可以利用真菌rRNA基因ITS2区域的扩增子测序来分析表层和亚表层沙子中的真菌群落。土壤表层真菌群落以子囊菌门为主（43.5 ~ 75.6%）。刮刮后，在建立的过滤器中发现了高丰度（>70%）的罗氏菌门。这些真菌也在接种过的过滤器中检测到，但在未接种过的过滤器中没有检测到，这表明通过接种可能会扩散到新的过滤器中。不同的Rozellomycota序列可能代表6个不同的目级分支，其中大多数与先前观察到的分支03 Rozellomycota有关。它们在SSF功能中的作用尚不清楚，但可能与指示菌的去除有关，因为该门包括放牧真核生物的潜在寄生虫。已知构成微生物风险或有助于微污染物生物降解的真菌丰度很低，只是偶尔检测到。SSF生物膜中61.8%的真菌可以预测生活方式性状；其中大多数是腐坏性微真菌或酵母。本研究概述了全尺寸SSF中真菌群落的组成及其与水质的潜在相互作用。它还强调需要更多关于“暗物质”真菌生态学的知识，如罗泽洛菌，并为这些微生物的未来研究提供了一个可访问和社会相关的环境。

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Uncharacterized members of the phylum Rozellomycota dominate the fungal community of a full-scale slow sand filter for drinking water production.

Slow sand filters (SSFs) for drinking water production are habitats for diverse microbes from multiple domains of life, which are integral to the ability of SSFs to purify water. While cultivation-independent analyses of the prokaryotic communities of SSFs have provided valuable insights, little attention has been paid to fungi inhabiting SSFs. This study characterized the fungal communities in the sand biofilm of one established, one inoculated and one non-inoculated SSF. The removal of the top-layer of sand (“scraping”) allowed fungal communities in the top and subsurface layers of sand to be analyzed using amplicon sequencing of the ITS2 region of fungal rRNA genes.

The top layers of SSF sand contained fungal communities dominated by phylum Ascomycota (43.5–75.6 %). After scraping, high abundances (>70 %) of phylum Rozellomycota were revealed in the established filter. These fungi were also detected in an inoculated filter, but not in a non-inoculated filter, suggesting potential dispersal to new filters by inoculation. The diverse Rozellomycota sequences potentially represented 6 different order-level clades, with most being related to previously observed Branch03 Rozellomycota. Their roles in SSF function are unknown but may be related to the removal of indicator bacteria as this phylum includes potential parasites of grazing eukaryotes. Fungi known to constitute microbial risk or contribute to micropollutant biodegradation were in low abundance and only sporadically detected. Lifestyle traits could be predicted for 61.8 % of fungi in the SSF biofilm; most of these were saprotrophic microfungi or yeasts.

This study presents an overview of the composition of fungal communities in full-scale SSF, and their potential interactions with water quality. It also highlights the need for more knowledge regarding the ecology of “dark matter”-fungi, such as Rozellomycota, and presents an accessible and societally relevant environment for future research of these microbes.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.