物种特异性环状RNA circDS-1通过调控二态转变促进马尔尼菲Talaromyces marneffei的适应性进化。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-03-06 eCollection Date: 2025-03-01 DOI:10.1371/journal.pgen.1011482
Xueyan Hu, Minghao Du, Changyu Tao, Juan Wang, Yun Zhang, Yueqi Jin, Ence Yang
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引用次数: 0

摘要

热适应性是哺乳动物病原真菌的一个重要特征。热二态真菌已经进化出一种独特的能力,通过从菌丝转变为酵母来响应宿主的体温。这些真菌和它们的近亲之间的蛋白质编码基因高度相似,这表明非编码元件在真菌热适应中不可或缺但经常被忽视的作用。在这里,我们系统地描绘了Talaromyces marneffei(一种典型的引起致命talaromyosis的热二态真菌)的菌丝和酵母条件下的全长环状rna的景观,通过优化利用第二代和第三代测序的circRNA检测的整合管道。我们发现T. marneffei circRNA具有长度较短、丰度较低和循环偏倚剪接等特征。然后,我们发现并验证了circDS-1,独立于其亲本基因,促进菌丝到酵母的转变,维持酵母形态,并参与毒力调节。进一步的分析和实验证实,circDS-1的产生是由circDS-1侧翼内含子中的T. marneffei特异性区域驱动的。总之,我们的发现不仅为circRNA在真菌热适应中的作用提供了新的见解,而且揭示了源自内含子突变的功能性circRNA的适应性进化的新的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Species-specific circular RNA circDS-1 enhances adaptive evolution in Talaromyces marneffei through regulation of dimorphic transition.

Thermal adaptability is a crucial characteristic for mammalian pathogenic fungi that originally inhabit natural ecosystems. Thermally dimorphic fungi have evolved a unique ability to respond to host body temperature by shifting from mycelia to yeast. The high similarity of protein-coding genes between these fungi and their relatives suggests the indispensable but often overlooked roles of non-coding elements in fungal thermal adaptation. Here, we systematically delineated the landscape of full-length circRNAs in both mycelial and yeast conditions of Talaromyces marneffei, a typical thermally dimorphic fungus causing fatal Talaromycosis, by optimizing an integrative pipeline for circRNA detection utilizing next- and third-generation sequencing. We found T. marneffei circRNA demonstrated features such as shorter length, lower abundance, and circularization-biased splicing. We then identified and validated that circDS-1, independent of its parental gene, promotes the hyphae-to-yeast transition, maintains yeast morphology, and is involved in virulence regulation. Further analysis and experiments among Talaromyces confirmed that the generation of circDS-1 is driven by a T. marneffei-specific region in the flanking intron of circDS-1. Together, our findings not only provide fresh insights into the role of circRNA in fungal thermal adaptation but also reveal a novel molecular mechanism for the adaptive evolution of functional circRNAs derived from intronic mutations.

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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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