基于TaqMan探针的多重实时定量pcr同时检测小鼠、啮齿动物和鼩鼱携带的克布病毒、乙型疟蚊病毒、乙型罗萨韦病毒和猴病毒。

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-03-06 DOI:10.1186/s12985-025-02671-4

Shunchang Fan, Minyi Zhang, Yucheng Li, Jingli Tian, Juxian Xian, Qing Chen

{"title":"基于TaqMan探针的多重实时定量pcr同时检测小鼠、啮齿动物和鼩鼱携带的克布病毒、乙型疟蚊病毒、乙型罗萨韦病毒和猴病毒。","authors":"Shunchang Fan, Minyi Zhang, Yucheng Li, Jingli Tian, Juxian Xian, Qing Chen","doi":"10.1186/s12985-025-02671-4","DOIUrl":null,"url":null,"abstract":"Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples. The approach demonstrated high sensitivity and specificity, with detection limits of 1 × 102 copies/µL for kobuvirus, parechovirus B, and rosavirus B, and 50 copies/µL for hunnivirus. Evaluation using 149 clinical samples showed strong concordance with conventional PCR methods.Conclusions: This work developed an effective multiplex qPCR method for the simultaneous detection of emerging picornaviruses particularly in rodents, including kobuvirus, parechovirus B, rosavirus B, and hunnivirus. Our findings contribute valuable insights into the monitoring and prevention of zoonotic diseases associated with these pathogens.","PeriodicalId":23616,"journal":{"name":"Virology Journal","volume":"22 1","pages":"61"},"PeriodicalIF":4.0000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883910/pdf/","citationCount":"0","resultStr":"{\"title\":\"A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.\",\"authors\":\"Shunchang Fan, Minyi Zhang, Yucheng Li, Jingli Tian, Juxian Xian, Qing Chen\",\"doi\":\"10.1186/s12985-025-02671-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples. The approach demonstrated high sensitivity and specificity, with detection limits of 1 × 102 copies/µL for kobuvirus, parechovirus B, and rosavirus B, and 50 copies/µL for hunnivirus. Evaluation using 149 clinical samples showed strong concordance with conventional PCR methods.Conclusions: This work developed an effective multiplex qPCR method for the simultaneous detection of emerging picornaviruses particularly in rodents, including kobuvirus, parechovirus B, rosavirus B, and hunnivirus. Our findings contribute valuable insights into the monitoring and prevention of zoonotic diseases associated with these pathogens.\",\"PeriodicalId\":23616,\"journal\":{\"name\":\"Virology Journal\",\"volume\":\"22 1\",\"pages\":\"61\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-03-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883910/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virology Journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12985-025-02671-4\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12985-025-02671-4","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景：小核糖核酸病毒是人类和各种动物中常见的传染性病原体，对健康构成重大威胁。传统的单链PCR广泛应用于实验室诊断，但相对耗时和费力。结果：建立了一种基于多重TaqMan探针的实时定量PCR （qPCR）方法，可同时快速检测鼠、鼠、鼠和鼩鼱样本中的克布病毒、帕库病毒B、罗萨维病毒B和猴病毒。该方法具有较高的灵敏度和特异性，对科布病毒、B型parechovirus和B型rosavvirus的检出限为1 × 102 copies/µL，对牛痘病毒的检出限为50 copies/µL。对149份临床样本进行评估，结果显示与传统PCR方法具有很强的一致性。结论：本工作建立了一种有效的多重qPCR方法，用于同时检测新出现的小核糖核酸病毒，特别是在啮齿动物中，包括kobuvirus， parechovirus B， rosavvirus B和hunnivirus。我们的发现为与这些病原体相关的人畜共患疾病的监测和预防提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

A TaqMan probe-based multiplex real-time quantitative pcr for simultaneous detection of kobuvirus, parechovirus B, rosavirus B, and hunnivirus carried by murine rodents and shrews.

Background: Picornaviruses, common infectious agents in humans and various animal species, pose significant health threats. Conventional monoplex PCR is widely employed in laboratory diagnostics but is relatively time-intensive and laborious.

Results: In this study, we developed a multiplex TaqMan probe-based real-time quantitative PCR (qPCR) assay for the rapid and simultaneous detection of kobuvirus, parechovirus B, rosavirus B and hunnivirus in murine rodent and shrew samples. The approach demonstrated high sensitivity and specificity, with detection limits of 1 × 10² copies/µL for kobuvirus, parechovirus B, and rosavirus B, and 50 copies/µL for hunnivirus. Evaluation using 149 clinical samples showed strong concordance with conventional PCR methods.

Conclusions: This work developed an effective multiplex qPCR method for the simultaneous detection of emerging picornaviruses particularly in rodents, including kobuvirus, parechovirus B, rosavirus B, and hunnivirus. Our findings contribute valuable insights into the monitoring and prevention of zoonotic diseases associated with these pathogens.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.