分子遗传学揭示了两种共属蜗牛吸虫的宿主特异性。

IF 1 4区 医学 Q4 PARASITOLOGY
Sandy Rey B Bradecina, Osamu Miura
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引用次数: 0

摘要

宿主特异性对于了解寄生虫的生态学和进化至关重要,由于存在形态相似但遗传上不同的物种,因此通常难以估计。由于缺乏仅出现在性成熟成虫阶段的诊断特征,幼虫吸虫的形态学鉴定往往具有挑战性。准确物种鉴定的困难可能会模糊它们与宿主物种的相容性。我们研究了两种亚洲泥螺(多形巴提拉利亚和白斑巴提拉利亚)的寄生虫,在两种寄主蜗牛共存的一个地点,以确定它们的寄主特异性水平。结果表明,双歧螺旋体和多形体螺旋体中最常见的吸虫种是形态种双歧螺旋体。采用聚合酶链反应(PCR)技术对77例双歧杆菌(B. atamentaria)和150例多形双歧杆菌(B. multiformis)感染的batillariae进行了限制性片段长度多态性(RFLP)分析。我们进一步对具有代表性的RFLP模式进行测序,以评估它们的系统发育关系。我们观察到6个遗传上不同的物种感染2个宿主物种。其中2种寄生虫只感染双歧螺旋体,1种寄生虫只感染多形虫。虽然有3种寄生虫同时感染两种宿主,但其中2种寄生虫对任何一种宿主都表现出高度偏向性感染。我们的研究表明,螺蛳中的吸虫可能具有宿主特异性,并强调了分子遗传学在评估宿主特异性方面的有用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MOLECULAR GENETICS UNVEIL HOST SPECIFICITY OF TREMATODES IN TWO COGENERIC BATILLARIA SNAILS.

Host specificity is essential to understanding the ecology and evolution of parasites, and it is often complicated to estimate because of the presence of morphologically similar but genetically distinct species. Morphological identification of larval trematodes is often challenging because of the lack of diagnostic characteristics that only appear in the sexually mature adult stage. The difficulty in accurate species identification may obscure their compatibility with the host species. We investigate the parasites infecting 2 Asian mud snail species, Batillaria multiformis and Batillaria attramentaria, at 1 site where the 2 host snails co-occur to identify the level of their host specificity. We found that the morphospecies Cercaria batillariae was the most common trematode species in B. attramentaria and B. multiformis. We conducted polymerase chain reaction (PCR) -based restriction fragment-length polymorphism (RFLP) analyses of 77 C. batillariae infections in B. attramentaria and 150 in B. multiformis. We further sequenced the representative RFLP patterns to evaluate their phylogenetic relationship. We observed 6 genetically distinct species infecting 2 host species. Of those, 2 parasite species exclusively infected B. attramentaria, and 1 was found only in B. multiformis. Although 3 parasite species infected both hosts, 2 of them showed highly biased infections to either host species. Our study suggests that trematodes in the Batillaria snails can be host specific and highlighted the usefulness of molecular genetics for assessing host specificity.

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来源期刊
Journal of Parasitology
Journal of Parasitology 医学-寄生虫学
CiteScore
2.10
自引率
7.70%
发文量
60
审稿时长
2 months
期刊介绍: The Journal of Parasitology is the official peer-reviewed journal of the American Society of Parasitologists (ASP). The journal publishes original research covering helminths, protozoa, and other parasitic organisms and serves scientific professionals in microbiology, immunology, veterinary science, pathology, and public health. Journal content includes original research articles, brief research notes, announcements of the Society, and book reviews. Articles are subdivided by topic for ease of reference and range from behavior and pathogenesis to systematics and epidemiology. The journal is published continuously online with one full volume printed at the end of each year.
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