唾液腺屏蔽:长得rhodus唾液腺提取物减少肠道免疫病理,并防止弓形虫感染。

IF 4.3 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Roberto Augusto Pereira Sousa, Jean Henrique Nunes de Paula, Rafaela José Silva, Samuel Cota Teixeira, Flávia Batista Ferreira França, Amanda Helena Leão Gonçalves, Túlio Rodrigues Oliveira Silva, Maria Julia Granero-Rosa, Murilo Vieira Silva, Marcos de Lucca Moreira Gomes, Marcos Vinícius Silva, Virmondes Rodrigues Junior, José Roberto Mineo, Bellisa Freitas Barbosa, Eloisa Amália Vieira Ferro, Carlo José Freire Oliveira, Angelica Oliveira Gomes
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引用次数: 0

摘要

C57BL/6小鼠经口感染弓形虫后,出现明显的严重肠道炎症,引起坏死、体重减轻和细菌易位。此外,长毛鼠唾液中还存在免疫调节分子,如脂钙素、硝基磷素和脲酶。我们的目的是评估长尾红唾液腺提取物(SGE)对经口感染弓形虫的小鼠的免疫调节作用。实验组在慢性感染期不给药(PBS),急性感染期不给药(30µg);对照组不感染,用SGE(30µg)治疗或不治疗。每天腹腔注射SGE,第3天灌胃感染小鼠20个刚地弓形虫(ME-49株)囊。实验治疗时间为慢性感染期23天(对应感染期20天),急性感染期12天(对应感染期9天)。经sge治疗的小鼠显示小肠缩短、体重减轻、临床评分和更高的存活率。处理后的小鼠肠道中调节和保护细胞因子(IL-4、IL-2、IL-10、IL-22)的分泌增加,IL-4(慢性期)、IL-2和IL-22(急性期)水平升高。SGE处理(30µg)对刚地弓形虫感染小鼠的十二指肠和回肠均有保护作用。接受治疗的动物表现出保存较好的绒毛结构,增加了杯状细胞和潘氏细胞计数,隐窝变浅。相关数据显示,接受治疗的动物表现出更受调节和保护性的免疫反应。总的来说,SGE有助于保持肠道完整性和减少炎症。因此,我们得出结论,SGE诱导了调节反应,减轻了炎症并保护了弓形虫感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Salivary shield: Rhodnius prolixus salivary glandular extract reduces intestinal immunopathology and protects against Toxoplasma gondii infection.

C57BL/6 mice, orally infected with T. gondii, experience pronounced severe intestinal inflammation, causing necrosis, weight loss, and bacterial translocation. In addition, immunomodulatory molecules such as lipocalins, nitrophorins, and apyrases are present in R. prolixus saliva. Our objective was to assess the immunomodulatory effects of the salivary gland extract (SGE) of R. prolixus in mice orally infected by T. gondii. Experimental groups received no treatment (PBS) or SGE (10 µg and 30 µg) in the chronic infection phase and (30 µg) in the acute infection phase. Control groups were non-infected and treated or not treated with SGE (30 µg). SGE was injected intraperitoneally daily, and mice were infected by gavage with 20 cysts of T. gondii (ME-49 strain) on the third treatment day. The treatment duration for the experiment was 23 days for the chronic infection phase (corresponding to 20 days of infection) and 12 days for the acute infection phase (corresponding to 9 days of infection). SGE-treated mice showed reduced small intestine shortening, weight loss, clinical scores, and higher survival rates. Treated mice also exhibited increased secretion of regulatory and protective cytokines (IL-4, IL-2, IL-10, IL-22) and higher levels of IL-4 (chronic phase), IL-2, and IL-22 (acute phase) in the gut. SGE treatment (30 µg) demonstrated protective effects in both the duodenum and ileum of T. gondii-infected mice. Treated animals showed better-preserved villus architecture, increased goblet and Paneth cell counts, and shallower crypts. Correlation data revealed that treated animals exhibited a more regulated and protective immune response. Overall, SGE contributed to the preservation of intestinal integrity and the reduction of inflammation. Thus, we conclude that SGE induces a regulatory response, mitigating inflammation and protecting against T. gondii infection.

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来源期刊
Gut Pathogens
Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY
CiteScore
7.70
自引率
2.40%
发文量
43
期刊介绍: Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).
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