魔芋通过调节PI3K/AKT细胞信号通路抑制雌激素受体+ (ER+)乳腺癌进展的药理作用

IF 2.3 4区 医学 Q3 ONCOLOGY
Hailong Li, Qinghong Yu, Jiaqing Song, Haining Ding, Yian Chen, Ying Jin, Hongting Wu, Liaqat Hussain, Xiufei Gao
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引用次数: 0

摘要

乳腺癌(Breast Cancer, BC)是女性最常见的恶性肿瘤之一。雌激素受体阳性(ER+)乳腺癌的发病率高达70%,且呈上升趋势。魔芋根茎(APR)具有治疗乳腺癌的潜力。目的:本研究旨在探讨不同APR提取物对ER+BC增殖、迁移和侵袭的影响,并从分子水平探讨其可能的机制。方法:用石油醚、乙酸乙酯、正丁醇、水等不同溶剂制备黄芪提取物。获得ER+ T47D乳腺癌细胞株,评价APR提取物对ER+ BC的影响。采用细胞计数试剂盒(CCk8)法评估细胞活力,采用transwell法和伤口愈合法检测抗侵袭和迁移作用。对所有提取物进行初步筛选,发现乙酸乙酯部分(APR-EA)最有效。超高效液相色谱(UHPLC)分析表明,黄芪提取物中含有琥珀酸、2-甲氧基间苯二酚、青霉酸、吗啡、水杨酸、α-亚麻酸、亚麻酸等多种植物化学物质。采用流式细胞术、免疫印迹和免疫组织化学方法探讨其分子机制。结果:APR-EA对ER+ T47D细胞株具有抗增殖、抗迁移和抗侵袭作用。因此,APR-EAE可能抑制P-PI3K/PI3K和P-Akt/Akt蛋白的表达,从而抑制ERα的表达。这种抑制作用影响下游蛋白CDK4和Bcl-2的表达,这些蛋白与细胞生长和凋亡有关。结论:APR-EA可能增加P21和Bax蛋白的表达,而P21和Bax蛋白与细胞周期阻滞和凋亡有关。总的来说,这些作用有助于APR-EA的抗er +乳腺癌特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pharmacological Evaluation of Amorphophalli rhizoma to Inhibit the Progression of Estrogen Receptor+ (ER+) Breast Cancer by Modulating the PI3K/AKT Cell Signaling Pathway.

Introduction: Breast Cancer (BC) is one of the most prevalent malignant tumors in women. The incidence of estrogen receptor-positive (ER+) breast cancer is as high as 70%, and it is increasing. Amorphophalli rhizoma (APR) has the potential to be used in breast cancer.

Aims: The objectives of the present study were to explore the impact of different APR extracts on the proliferation, migration, and invasion of ER+BC and to investigate their possible mechanism at the molecular level.

Methods: Various extracts of APR were prepared in different solvents, such as petroleum ether, ethyl acetate, n-butanol, and water. ER+ T47D breast cancer cell lines were acquired and uti-lized to assess the effect of APR extracts on ER+ BC. Cell viability was assessed using the cell counting kit8 (CCk8) method, while anti-invasive and migratory effects were examined by transwell and wound healing assay. All the extracts were initially screened, and the ethyl acetate fraction (APR-EA) was found to be the most effective. Ultra High-Performance Liquid Chro-matography (UHPLC) of APR-EAE extract revealed the presence of various phytochemicals, such as succinic acid, 2-methoxy resorcinol, penicillic acid, morphine, salicylic acid, α-linolenic acid, and linolenic acid. Flow cytometry, western blot, and immunohistochemistry were used to explore molecular mechanisms.

Results: APR-EA demonstrated anti-proliferative, anti-migratory, and anti-invasive effects on the ER+ T47D cell line. Thus, APR-EAE might inhibit the expression of P-PI3K/PI3K and P-Akt/Akt proteins, which subsequently represses the expression of ERα. This inhibition affects the downstream expression of the proteins CDK4 and Bcl-2, which are linked to cell growth and apoptosis.

Conclusion: Additionally, APR-EA might increase the expression of P21 and Bax proteins, which are associated with cell cycle arrest and apoptosis. Overall, these effects contribute to the anti-ER+ breast cancer properties of APR-EA.

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来源期刊
Current cancer drug targets
Current cancer drug targets 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
105
审稿时长
1 months
期刊介绍: Current Cancer Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular drug targets involved in cancer, e.g. disease specific proteins, receptors, enzymes and genes. Current Cancer Drug Targets publishes original research articles, letters, reviews / mini-reviews, drug clinical trial studies and guest edited thematic issues written by leaders in the field covering a range of current topics on drug targets involved in cancer. As the discovery, identification, characterization and validation of novel human drug targets for anti-cancer drug discovery continues to grow; this journal has become essential reading for all pharmaceutical scientists involved in drug discovery and development.
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