摘要
引言 短链脂肪酸（SCFAs）是一种小分子代谢产物，主要由肠道中的膳食纤维经微生物发酵产生，已被证明对人体健康有益。本研究旨在评估 SCFA 对人体骨骼肌能量代谢的影响。 方法 在有无 SCFAs 的情况下，对原代人体肌管的葡萄糖和脂肪酸（油酸）代谢以及胰岛素敏感性和蛋白质合成进行分析。 结果 在丁酸盐处理后，观察到 SCFAs 对 14C - 油酸摄取和氧化以及 14C - 亮氨酸摄取和蛋白质合成的最明显影响。丁酸盐使 14C 亮氨酸的积累增加了两倍，这可能是由于蛋白质的结合。另一方面，丁酸盐将 14C 亮氨酸转化为游离脂肪酸的过程减少了 50%以上。14C-acetate 和 14C-butyrate 均被原代人类肌管吸收和利用。没有发现 SCFAs 会影响葡萄糖代谢或胰岛素效应。 结论 目前的研究结果表明，在 SCFAs 中，丁酸盐是调节原发性人体肌管代谢最有效的 SCFAs。
Introduction
Short-chain fatty acids (SCFAs) are small molecule metabolites mainly produced during microbial fermentation of dietary fibre in the gut and have been shown to have a beneficial impact on human health. The aim of this study was to evaluate the effect of SCFAs on human skeletal muscle energy metabolism.
Methods
Primary human myotubes were analysed for glucose and fatty acid (oleic acid) metabolism, as well as insulin sensitivity and protein synthesis in the presence or absence of SCFAs.
Results
The most pronounced effects of SCFAs were observed on 14C-oleic acid uptake and oxidation, as well as 14C-leucine uptake and protein synthesis, following butyrate treatment. Butyrate increased 14C-leucine accumulation twofold, potentially due to protein incorporation. On the other hand, the conversion of 14C-leucine into free fatty acids was reduced by more than 50% by butyrate. Both 14C-acetate and 14C-butyrate were shown to be taken up and utilised by primary human myotubes. None of the SCFAs were found to influence glucose metabolism or insulin effects.
Conclusion
The results from the current study thus suggest that among the SCFAs, butyrate emerges as the most powerful SCFA in regulating primary human myotube metabolism.
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