医院污水中病原微生物的分析：了解抗生素耐药性和环境健康风险的统计方法

Q1 Environmental Science

Environmental Nanotechnology, Monitoring and Management Pub Date : 2025-03-05 DOI:10.1016/j.enmm.2025.101059

Fatimazahra Sayerh , Latifa Mouhir , Laila Saafadi , Ilham Nassri , Abdelmoula El Ouardi , Najia Ameur

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引用次数: 0

摘要

抗生素耐药性是防治传染病的一个重大公共卫生问题，医院废水是一类特殊的液体废物，因其传染性和毒性而具有危险性。然而，这些废水正变得无法控制，它们排放到环境中的问题变得越来越重要，特别是因为医院废水是抗生素细菌的来源。本研究旨在分析摩洛哥西南地区（Temara-Sale镇）两家地市级医院的医院污水，通过每周现场抽样，评估这些污水中微生物质量和耐抗生素细菌的多样性。从两个地点采集的样本显示，粪便指示细菌和病原体的负荷很高，特别是总大肠菌群，其水平范围为102和7.5 × 104 UFC/100 ml，粪便大肠菌群（8,1 × 106 UFC/100 ml），大肠杆菌（5,1 × 106 UFC/100 ml），肠球菌（8,1 × 103 UFC/100 ml），金黄色葡萄球菌（6,6 × 106 UFC/100 ml）和铜绿假单胞菌（7,9 × 106 UFC/100 ml）。对从医院流出物中分离的菌株的抗生素耐药性的研究表明，在检查的75株分离物中，大肠杆菌是两家医院中最常检测到的分离物，PHT医院的流行率为42%，PHS医院的流行率为47%。其次是肺炎克雷伯菌、铜绿假单胞菌和金黄色葡萄球菌，在PHT医院的患病率分别为29%、16%和12%，在PHS医院的患病率分别为16%、22%和13%。结果显示对不同抗菌素的耐药率不同，对β -内酰胺类抗生素的耐药率较高。所研究的两家医院的医院污水特征表明，这些污水存在健康和环境风险，使医院-环境界面成为有利于抗性转移的地方，因此迫切需要在排放到自然环境之前制定具体的处理方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analysis of pathogenic microorganisms in hospital effluents: A statistical approach to understanding antibiotic resistance and environmental health risks

查看原文本刊更多论文

Analysis of pathogenic microorganisms in hospital effluents: A statistical approach to understanding antibiotic resistance and environmental health risks

Antibiotic resistance is a significant public health issue in the fight against infectious diseases, and hospital effluent is a special category of liquid waste, hazardous because of its contagious and toxic characteristics. However, these effluents are becoming uncontrollable, and the problem of their discharge into the environment is becoming increasingly important, especially as hospital effluents are a source of antibiotic bacteria. This work aims to analyze hospital effluents from two prefectural hospitals in the southwest region of Morocco (Temara-Sale towns) by assessing the microbiological quality and diversity of antibiotic-resistant bacteria in these effluents collected via weekly spot sampling. Samples taken from both sites showed high loads of fecal indicator bacteria and pathogens, particularly total coliforms, with levels ranging from 10² and 7,5 × 10⁴ UFC/100 ml, fecal coliforms (8,1 × 10⁶ UFC/100 ml), Escherichia coli (5,1 × 10⁶ UFC/100 ml), intestinal enterococci (8,1 × 10³ UFC/100 ml), Staphylococcus aureus (6,6 × 10⁶ UFC/100 ml) and Pseudomonas aeruginosa (7,9 × 10⁶ UFC/100 ml). The study of antibiotic resistance in strains isolated from hospital effluent revealed that of the 75 isolates examined, Escherichia coli was the most commonly detected isolate in both hospitals, with a prevalence of 42 % at PHT Hospital and 47 % at PHS Hospital. It was followed by Klebsiella pneumonia, Pseudomonas aeruginosa, and Staphylococcus aureus, with respective prevalences of 29 %, 16 %, and 12 % at PHT Hospital, and 16 %, 22 % and 13 % at PHS Hospital. The results show varying resistance rates to different antimicrobials, with high levels of resistance observed with antibiotics belonging to the beta-lactam class. Characterization of the hospital effluents from the two hospitals studied showed that these effluents present health and environmental risks that qualify the hospital-environment interface as a place conducive to the transfer of resistance, thus necessitating the urgent development of specific treatment methods before discharge into the natural environment.

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来源期刊

Environmental Nanotechnology, Monitoring and Management Environmental Science-Water Science and Technology

CiteScore

13.00

自引率

0.00%

发文量

132

审稿时长

48 days

期刊介绍： Environmental Nanotechnology, Monitoring and Management is a journal devoted to the publication of peer reviewed original research on environmental nanotechnologies, monitoring studies and management for water, soil , waste and human health samples. Critical review articles, short communications and scientific policy briefs are also welcome. The journal will include all environmental matrices except air. Nanomaterials were suggested as efficient cost-effective and environmental friendly alternative to existing treatment materials, from the standpoints of both resource conservation and environmental remediation. The journal aims to receive papers in the field of nanotechnology covering; Developments of new nanosorbents for: •Groundwater, drinking water and wastewater treatment •Remediation of contaminated sites •Assessment of novel nanotechnologies including sustainability and life cycle implications Monitoring and Management papers should cover the fields of: •Novel analytical methods applied to environmental and health samples •Fate and transport of pollutants in the environment •Case studies covering environmental monitoring and public health •Water and soil prevention and legislation •Industrial and hazardous waste- legislation, characterisation, management practices, minimization, treatment and disposal •Environmental management and remediation