综合转录组和代谢组分析揭示了两种山茶品种对金弓翅虫(半翅目:蚜虫科)的反应。

Jinjuan Fan, Xiao Zhang, Wenbin Jiang, Jin Xu, Mengyang Wu, Xinlong Dai, Fulin Xu, Suzhen Niu, Yingqin He
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引用次数: 0

摘要

茶蚜(半翅目:蚜虫科)是一种危害茶树的破坏性害虫。茶树对金黄色葡萄球菌侵染的抗性机制在很大程度上尚未被探索。以抗蚜的茶树品种“前茶1号”(QC1)和对蚜敏感的茶树品种“黄金芽”(HJY)为研究对象,研究了茶树对金田鼠取食的防御反应。对4个样本进行转录组学和代谢组学分析:QCCK (T. aurantii未侵染的QC1)、HJYCK (T. aurantii未侵染的HJY)、QC24 (T. aurantii侵染的QC1)和HJY24 (T. aurantii侵染的HJY)的差异表达基因(DEGs)和差异积累代谢物(DAMs)在2个对照组(QCCK vs. QC24和HJYCK vs. HJY24)中主要富集在激素信号转导、苯丙素生物合成和类黄酮生物合成等代谢途径上。蚜虫侵染后,抗性品种QC1比敏感品种HJY表现出更多的deg和DAMs,表明抗性品种对金蝇摄食胁迫的响应更强。此外,与敏感品种HJY相比,抗性品种QC1中苯丙素和类黄酮相关基因(CYP、4CL、FLS、F3H和LAR)的表达显著上调。参与苯丙素/类黄酮途径的代谢物,如对香豆酰辅酶a、咖啡酰奎宁酸和阿铁酰辅酶a,在QC1中被完全诱导。这些结果表明,苯丙素/黄酮类化合物途径在茶树抗金曲菌侵染过程中发挥了关键作用,为抗性种质资源的选育和利用提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrative transcriptome and metabolome analysis uncovers the Toxoptera aurantii (Hemiptera: Aphididae) response of two Camellia sinensis (Ericales: Theaceae) cultivars.

The tea aphid Toxoptera aurantii Boyer (Hemiptera: Aphididae) is a destructive pest that infests tea plants. The resistance mechanisms of the tea plant against T. aurantii infestation are largely unexplored. This study investigates the defensive response of tea plants to T. aurantii feeding using an aphid-resistant Camellia sinensis cultivar 'Qiancha1' (QC1) and an aphid-susceptible C. sinensis cultivar 'Huangjinya' (HJY). Transcriptomics and metabolomics analyses were conducted on 4 samples: QCCK (T. aurantii non-infested QC1), HJYCK (T. aurantii non-infested HJY), QC24 (T. aurantii-infested QC1 for 24 h), and HJY24 (T. aurantii-infested HJY for 24 h). The results showed that the differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in the 2 comparison groups (QCCK vs. QC24 and HJYCK vs. HJY24) were primarily enriched in metabolic pathways, including hormone signal transduction, phenylpropanoid biosynthesis and flavonoid biosynthesis. Following aphid infestation, the resistant cultivar QC1 exhibited more DEGs and DAMs than the susceptible cultivar HJY, indicating a stronger response to T. aurantii feeding stress. Additionally, the expression of phenylpropanoid- and flavonoid-related genes (CYP, 4CL, FLS, F3H, and LAR) was significantly upregulated in the resistant cultivar QC1 compared with that in the susceptible cultivar HJY. Metabolites involved in phenylpropanoid/flavonoid pathways, such as p-coumaroyl-CoA, caffeoylquinic acid, and feruloyl-CoA, were exclusively induced in QC1. These findings suggest that phenylpropanoid/flavonoid pathways play pivotal roles in tea plant resistance to T. aurantii infestation, providing valuable insights for the breeding and utilization of resistant germplasms.

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