阶梯式洗脱和电喷雾电离方式对土壤溶解有机质的化学多样性有互补作用

IF 2.6 3区 地球科学 Q2 GEOCHEMISTRY & GEOPHYSICS
Shasha Zhuo, Rong Qin, Qing-Long Fu
{"title":"阶梯式洗脱和电喷雾电离方式对土壤溶解有机质的化学多样性有互补作用","authors":"Shasha Zhuo,&nbsp;Rong Qin,&nbsp;Qing-Long Fu","doi":"10.1016/j.orggeochem.2025.104965","DOIUrl":null,"url":null,"abstract":"<div><div>The chemodiversity of dissolved organic matter (DOM) molecules can affect the biogeochemical cycling of carbon and nitrogen in terrestrial and aquatic environments. In this study, the negative and positive ion modes of electrospray ionization (ESI(−) and ESI(+), respectively) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) were employed to reveal the chemical composition of seven soil DOM isolates by stepwise elution using different methanol/water solvents. The intensity percentage of CHON molecules in soil DOM detected in the ESI(+) mode was 4.6%–47.3% higher than that for the ESI(−) mode. The O/C ratio, H/C ratio, and the nominal oxidation states of the carbon value of soil DOM molecules linearly correlated (<em>R</em><sup>2</sup> &gt; 0.8 for 72% number of stepwise eluted DOM isolates) with the increasing volume of methanol in the SPE elution solvents in dual ion modes, demonstrating the selective fractionation of oxygen-rich and highly polar molecules by methanol-poor solvents. This polarity-dependent fractionation is expected to be universal across different soils. These results suggest that combining the ESI(−) and ESI(+) modes with stepwise elution enables the comprehensive characterization of soil DOM composition, favouring the in-depth understanding of the critical roles of DOM in the biogeochemical cycles of carbon and nitrogen.</div></div>","PeriodicalId":400,"journal":{"name":"Organic Geochemistry","volume":"203 ","pages":"Article 104965"},"PeriodicalIF":2.6000,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemodiversity of soil dissolved organic matter complementally improved by stepwise elution and electrospray ionization modes\",\"authors\":\"Shasha Zhuo,&nbsp;Rong Qin,&nbsp;Qing-Long Fu\",\"doi\":\"10.1016/j.orggeochem.2025.104965\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The chemodiversity of dissolved organic matter (DOM) molecules can affect the biogeochemical cycling of carbon and nitrogen in terrestrial and aquatic environments. In this study, the negative and positive ion modes of electrospray ionization (ESI(−) and ESI(+), respectively) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) were employed to reveal the chemical composition of seven soil DOM isolates by stepwise elution using different methanol/water solvents. The intensity percentage of CHON molecules in soil DOM detected in the ESI(+) mode was 4.6%–47.3% higher than that for the ESI(−) mode. The O/C ratio, H/C ratio, and the nominal oxidation states of the carbon value of soil DOM molecules linearly correlated (<em>R</em><sup>2</sup> &gt; 0.8 for 72% number of stepwise eluted DOM isolates) with the increasing volume of methanol in the SPE elution solvents in dual ion modes, demonstrating the selective fractionation of oxygen-rich and highly polar molecules by methanol-poor solvents. This polarity-dependent fractionation is expected to be universal across different soils. These results suggest that combining the ESI(−) and ESI(+) modes with stepwise elution enables the comprehensive characterization of soil DOM composition, favouring the in-depth understanding of the critical roles of DOM in the biogeochemical cycles of carbon and nitrogen.</div></div>\",\"PeriodicalId\":400,\"journal\":{\"name\":\"Organic Geochemistry\",\"volume\":\"203 \",\"pages\":\"Article 104965\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-02-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Organic Geochemistry\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0146638025000385\",\"RegionNum\":3,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"GEOCHEMISTRY & GEOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Organic Geochemistry","FirstCategoryId":"89","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0146638025000385","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GEOCHEMISTRY & GEOPHYSICS","Score":null,"Total":0}
引用次数: 0

摘要

溶解有机质(DOM)分子的化学多样性影响着陆地和水生环境中碳氮的生物地球化学循环。本研究采用电喷雾电离的负离子模式(ESI(−)和正离子模式(ESI(+))结合傅里叶变换离子回旋共振质谱(FT-ICR MS),通过不同的甲醇/水溶剂逐步洗脱,揭示了7种土壤DOM分离物的化学成分。ESI(+)模式下土壤DOM中检测到的CHON分子强度百分比比ESI(−)模式高4.6% ~ 47.3%。O/C比、H/C比与土壤DOM分子标称氧化态碳值呈线性相关(R2 >;在双离子模式下,随着固相萃取溶剂中甲醇体积的增加,DOM分离物的分离率为0.8(72%),证明了贫甲醇溶剂对富氧和高极性分子的选择性分离。这种与极性有关的分馏可望在不同的土壤中普遍存在。上述结果表明,ESI(−)和ESI(+)模式与逐步洗脱相结合,可以全面表征土壤DOM的组成,有利于深入了解DOM在碳氮生物地球化学循环中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemodiversity of soil dissolved organic matter complementally improved by stepwise elution and electrospray ionization modes
The chemodiversity of dissolved organic matter (DOM) molecules can affect the biogeochemical cycling of carbon and nitrogen in terrestrial and aquatic environments. In this study, the negative and positive ion modes of electrospray ionization (ESI(−) and ESI(+), respectively) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) were employed to reveal the chemical composition of seven soil DOM isolates by stepwise elution using different methanol/water solvents. The intensity percentage of CHON molecules in soil DOM detected in the ESI(+) mode was 4.6%–47.3% higher than that for the ESI(−) mode. The O/C ratio, H/C ratio, and the nominal oxidation states of the carbon value of soil DOM molecules linearly correlated (R2 > 0.8 for 72% number of stepwise eluted DOM isolates) with the increasing volume of methanol in the SPE elution solvents in dual ion modes, demonstrating the selective fractionation of oxygen-rich and highly polar molecules by methanol-poor solvents. This polarity-dependent fractionation is expected to be universal across different soils. These results suggest that combining the ESI(−) and ESI(+) modes with stepwise elution enables the comprehensive characterization of soil DOM composition, favouring the in-depth understanding of the critical roles of DOM in the biogeochemical cycles of carbon and nitrogen.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Organic Geochemistry
Organic Geochemistry 地学-地球化学与地球物理
CiteScore
5.50
自引率
6.70%
发文量
100
审稿时长
61 days
期刊介绍: Organic Geochemistry serves as the only dedicated medium for the publication of peer-reviewed research on all phases of geochemistry in which organic compounds play a major role. The Editors welcome contributions covering a wide spectrum of subjects in the geosciences broadly based on organic chemistry (including molecular and isotopic geochemistry), and involving geology, biogeochemistry, environmental geochemistry, chemical oceanography and hydrology. The scope of the journal includes research involving petroleum (including natural gas), coal, organic matter in the aqueous environment and recent sediments, organic-rich rocks and soils and the role of organics in the geochemical cycling of the elements. Sedimentological, paleontological and organic petrographic studies will also be considered for publication, provided that they are geochemically oriented. Papers cover the full range of research activities in organic geochemistry, and include comprehensive review articles, technical communications, discussion/reply correspondence and short technical notes. Peer-reviews organised through three Chief Editors and a staff of Associate Editors, are conducted by well known, respected scientists from academia, government and industry. The journal also publishes reviews of books, announcements of important conferences and meetings and other matters of direct interest to the organic geochemical community.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信