基于不依赖于支点的链位移反应网络的DNA逻辑电路

IF 9.1 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Junlan Liu*,  and , Qing Zhang, 
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引用次数: 0

摘要

DNA链位移在DNA纳米技术中被广泛应用于功能性DNA电路的编程。然而,许多这些系统依赖于单链DNA悬垂(支点)。尽管它很受欢迎,但消除对立足点的依赖将提高DNA电路的功能和实用性。在此,我们开发了一个用于DNA逻辑电路的不依赖于支点的DNA链位移(TISD)反应网络。TISD反应不是利用支点提供的焓,而是利用构型熵作为驱动力。研究了TISD的工作原理、设计框架和实际功能。基于tisd的DNA逻辑电路在级联、扇入和扇出信号转导等基本功能上表现出令人满意的性能。它们在数字计算方面也表现出相当的性能,包括布尔逻辑门、多层电路和平方根计算。TISD作为标准支点依赖系统的一个有希望的替代方案,将极大地扩展基于dna的分子编程的设计空间,并激发更多功能的基于dna的功能系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

DNA Logic Circuit Based on a Toehold-Independent Strand Displacement Reaction Network

DNA Logic Circuit Based on a Toehold-Independent Strand Displacement Reaction Network

DNA strand displacement is widely used in DNA nanotechnology for programming functional DNA circuits. However, many of these systems depend on a single-stranded DNA overhang (toehold). Despite its popularity, eliminating the reliance on a toehold will advance the functionality and practicality of DNA circuits. Herein we develop a toehold-independent DNA strand displacement (TISD) reaction network for DNA logic circuits. Instead of leveraging enthalpic energy provided by the toehold, the TISD reaction employs configurational entropy as the driving force. The working principle, design framework, and practical functionality of the TISD were investigated. TISD-based DNA logic circuits show desirable performances on basic functions like cascaded, fan-in, and fan-out signal transduction. They also exhibit comparable performance on digital computing, including Boolean logic gates, multilayer circuits, and square root computation. As a promising alternative to canonical toehold-dependent systems, TISD will largely expand the design space of DNA-based molecular programming and inspire more versatile DNA-based functional systems.

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来源期刊
Nano Letters
Nano Letters 工程技术-材料科学:综合
CiteScore
16.80
自引率
2.80%
发文量
1182
审稿时长
1.4 months
期刊介绍: Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.
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