纳米粒子介导的脂膜融合的物理决定因素

IF 5.8 3区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Nanoscale Pub Date : 2025-03-05 DOI:10.1039/d4nr04851b
Beatrice Leonardini, Davide Bochicchio, Paolo Volpe, Francesco Stellacci, Silvia Dante, Ester Canepa, Giulia Rossi, ANNALISA RELINI
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引用次数: 0

摘要

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Physical Determinants of Nanoparticle-mediated Lipid Membrane Fusion
A wide range of fundamental cellular activities rely on lipid membrane fusion. Membrane fusion processes can be mimicked by synthetic approaches to understand fusion mechanisms and develop novel drug delivery systems and therapeutic agents. Recently, membrane-embedded amphiphilic gold nanoparticles (AuNPs) have been employed as artificial fusogens to induce finely tuned membrane fusion in vitro. However, the physical determinants driving and regulating the fusion process mediated by AuNPs remain largely unexplored, thus limiting the application potential of this synthetic fusion system. Herein, we focus on unraveling the effect of the interplay between the curvature of the lipid membrane and the size of amphiphilic AuNPs during fusion events. We employed AuNPs with the same surface chemistry but different core diameters (~2 nm and ~4 nm) interacting with phosphatidylcholine unilamellar vesicles of different membrane curvatures containing a biologically relevant percentage of cholesterol. Based on a combination of fluorescence spectroscopy assays, dissipative quartz microbalance, and molecular dynamics simulations, our findings reveal that small AuNPs promote vesicle fusion regardless of the membrane curvature. In contrast, large AuNPs do not exhibit fusogenic properties with low curvature membranes, and can induce fusion events only with significantly curved membranes. Large NPs impede the progression from the stalk state to the hemifused state via steric hindrance, an effect that is only partially compensated by membrane curvature. These results offer novel insights into the role of AuNP core size and membrane curvature in mediating the interaction between the vesicles during fusion and highlight how understanding these physical determinants has broad implications in fully exploiting the application potential of novel synthetic fusion approaches.
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来源期刊
Nanoscale
Nanoscale CHEMISTRY, MULTIDISCIPLINARY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
12.10
自引率
3.00%
发文量
1628
审稿时长
1.6 months
期刊介绍: Nanoscale is a high-impact international journal, publishing high-quality research across nanoscience and nanotechnology. Nanoscale publishes a full mix of research articles on experimental and theoretical work, including reviews, communications, and full papers.Highly interdisciplinary, this journal appeals to scientists, researchers and professionals interested in nanoscience and nanotechnology, quantum materials and quantum technology, including the areas of physics, chemistry, biology, medicine, materials, energy/environment, information technology, detection science, healthcare and drug discovery, and electronics.
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