PDRG1作为前折叠蛋白样复合体的一个组成部分，对植物早期发育至关重要。

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2025-03-03 DOI:10.1002/1873-3468.70024

Laura Hernández-Villa, Alberto Palacios-Abella, Yaiza Gómez-Mínguez, Cecilia Costigliolo-Rojas, Eugenio G Minguet, David Alabadí

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引用次数: 0

摘要

p53和DNA损伤调节基因1 （PDRG1）是植物和动物中类似折叠蛋白前复合体（PFDLc）的一部分。PDRG1主要是作为PFDLc的一个亚基，还是作为一个独立的亚基，在任何真核生物中都不清楚。在这里，我们发现拟南芥中PDRG1活性的损伤会导致胚胎死亡，其他折叠蛋白样蛋白UXT和AtURI也是如此。PFDLc的亚基是体内PDRG1的主要相互作用体，PDRG1、UXT和AtURI的相互作用组表现出很强的重叠，包括核RNA聚合酶亚基和剪接体的各种复合物。我们的研究结果表明，PDRG1主要作为PFDLc的一个亚基在拟南芥中发挥重要作用。

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PDRG1 is essential for early plant development as a component of the prefoldin-like complex.

p53 AND DNA DAMAGE-REGULATED GENE1 (PDRG1) is part of the prefoldin-like complex (PFDLc) in plants and animals. Whether PDRG1 acts primarily as a subunit of PFDLc or as an independent subunit is not known in any eukaryote. Here, we show that impairment of PDRG1 activity in Arabidopsis thaliana leads to embryonic lethality, as is the case for the other prefoldin-like proteins UXT and AtURI. The subunits of PFDLc are the main interactors of PDRG1 in vivo, and the interactomes of PDRG1, UXT, and AtURI show strong overlaps, including subunits of nuclear RNA polymerases and various complexes of the spliceosome. Our results show that PDRG1 plays an essential role in Arabidopsis mainly as a subunit of PFDLc.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.