Algicidal mechanism and algicidal active metabolites of Alteromonas abrolhosensis against harmful dinoflagellates Karenia mikimotoi

Harmful algal blooms (HABs) are a global environmental concern, posing significant threats to marine ecosystems and human health. Algicidal bacteria offer a promising biological approach for mitigating HABs. Herein, the mechanism of an algicidal bacterium Alteromonas abrolhosensis JY-JZ1 against Karenia mikimotoi was investigated and algicidal metabolites from the strain JY-JZ1 were analyzed. The significant decrease in maximum quantum yield (Fv/Fm), relative electron transport rate (rETR), maximum relative electron transport rate (rETRmax) and apparent photosynthetic efficiency (α) indicated destruction of photosynthetic system. Biochemical analyses showed that the JY-JZ1 supernatant induced reactive oxygen species (ROS) overproduction and malondialdehyde (MDA) accumulation in K. mikimotoi. Contents of superoxide dismutase (SOD) and glutathione (GSH) increased responding to JY-JZ1 supernatant treatments. Optical microscope observation and propidium iodide (PI) staining confirmed the destruction of the cell membrane structure. Gene expression analysis showed that the extracellular metabolites of JY-JZ1 altered gene expression associated with photosynthesis, respiration, and cell wall integrity in K. mikimotoi. The metabolites of strain JY-JZ1 for 8 h and 24 h were harvested and analysed. Among the metabolites, 3-hydroxy-kynurenine, 10-undecenoic acid, 10-hydroxy-2-decenoic acid, 3-hydroxymandelic acid and catechol were first proved to exhibit algicidal activity against K. mikimotoi. This study provides the first report of these metabolites as novel algicidal substances. These results demonstrated that A. abrolhosensis JY-JZ1 exhibits significant potential for controlling HABs and offers multiple algicidal active compounds with promising application prospects.