有氧运动通过5-甲氧基色氨酸和P38MAPK调节动脉粥样硬化大鼠主动脉软骨形成和钙化。

IF 3.9

Experimental gerontology Pub Date : 2025-02-28 DOI:10.1016/j.exger.2025.112722

Liang-liang You , Xiao-bing Luo , Wen-qi Zhou , Rui-chi Zhang , Zhong-hao Li , Jia-xin Xu , Jing Ran , Jie Xu

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引用次数: 0

摘要

背景：5-甲氧基色氨酸（5-MTP）是一种新的内皮因子，具有血管保护和抗炎作用，可减少动脉粥样硬化期间主动脉软骨形成和钙化。本研究旨在探讨有氧运动对动脉粥样硬化大鼠主动脉软骨形成和钙化的影响。探讨有氧运动对5-MTP/P38 MAPK信号通路表达的影响。为运动对大鼠动脉粥样硬化模型的治疗作用奠定理论基础。方法：采用高脂饮食联合腹腔注射维生素D3建立动脉粥样硬化大鼠模型。有氧运动组在运动跑步机上进行中等强度有氧运动，持续8 周，动脉粥样硬化模型组和对照组不运动。运动后采集大鼠血液和主动脉标本，评估血清甘油三酯（TG）、胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDLC）、主动脉软骨形成、钙化、5-MTP水平、II型胶原、P38MAPK、pp38 MAPK和IL-6蛋白含量。结果：(1)8 周有氧运动可显著降低大鼠主动脉软骨形成、钙化面积、血清LDL-C、TC水平、动脉粥样硬化指数和血清IL-6水平（p ）结论：8 周有氧运动可改善AS大鼠血脂异常，降低主动脉软骨形成和钙化形成。其机制可能与增加主动脉5-MTP水平和抑制P38MAPK/ IL-6信号通路有关。

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Aerobic exercise modulates aortic chondrogenesis and calcification via 5-methoxytryptophan and P38MAPK in atherosclerotic rats

Background

5-Methoxytryptophan (5-MTP), a new endothelial factor with vasoprotective and anti-inflammatory effects, reduces aortic chondrogenesis and calcification during atherosclerosis. The aim of this study was to investigate the effects of aerobic exercise on aortic chondrogenesis and calcification during atherosclerosis in rats. To investigate the effect of aerobic exercise on the expression of 5-MTP/P38 MAPK signaling pathway. To lay a theoretical foundation for the therapeutic effect of exercise in rat atherosclerosis model.

Methods

Establishment of a rat model of atherosclerosis using a high-fat diet combined with intraperitoneal injection of vitamin D3 (VD3). The aerobic exercise group underwent moderate-intensity aerobic exercise on an exercise treadmill for 8 weeks, while the atherosclerosis model group and the control group did not exercise. After exercise, blood and aortic samples were collected from all rats to evaluate the levels of serum triglyceride (TG), cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDLC), aortic chondrogenesis, calcification, 5-MTP level, collagen II, P38MAPK, pp38 MAPK, and IL-6 protein content.

Results

(1)8 weeks of aerobic exercise significantly reduced aortic chondrogenesis, area of calcification, serum LDL-C, TC levels, atherosclerotic index and serum IL-6 levels in rats (p < 0.01), and lowered TG levels (p < 0.05);(2)8 weeks of aerobic exercise significantly increased aortic 5-MTP levels (p < 0.01) and decreased the content of aortic pp38MAPK, collagen II and IL-6 proteins significantly (p < 0.01). The pp38MAPK/P38MAPK ratio was also decreased (p < 0.01).

Conclusion

8 weeks of aerobic exercise training improved dyslipidemia and reduced aortic chondrogenesis and calcification formation in AS rats. The mechanism may be related to increasing aortic 5-MTP levels and inhibiting the P38MAPK/ IL-6 signaling pathway.

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来源期刊

Experimental gerontology Ageing, Biochemistry, Geriatrics and Gerontology

CiteScore

6.70

自引率

0.00%

发文量

审稿时长

66 days