TbCSV C3 蛋白中 C 端 7 个氨基酸基团的作用及其与 NbPOLA2 在增强病毒复制中的相互作用

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing
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引用次数: 0

摘要

烟草卷枝病毒(TbCSV)的C3蛋白是一种病毒复制增强子(REn),可能是介于真正的单株begomovirus和需要卫星分子才能感染的病毒之间的进化中间产物。然而,这种增强的机制在很大程度上是未知的。在这项研究中,我们产生了两个TbCSV的突变感染克隆:一个是C3基因的3'端区域缺失,该区域与C2不重叠(TbCSVdC3),另一个是该区域被一个系统发育无关的iLOV基因序列(TbCSVdC3-iLOV)所取代。我们的研究结果强调了C3的3′端区域在病毒DNA积累中的关键作用,并进一步证明了TbCSV C3蛋白在反式中的过表达弥补了TbCSVdC3的功能缺陷。进一步分析发现C3残基123-129的c端7个氨基酸基序在复制增强中起重要作用。先前的研究表明,其他一些双病毒编码的C3/AC3蛋白的分子内和分子间相互作用对它们增强复制的能力至关重要。有趣的是,在测试的潜在相互作用物中,DNA聚合酶α亚基NbPOLA2被证实在酵母和植物中与C3相互作用。我们的研究结果表明,NbPOLA2正调控TbCSV的复制和感染,并且C3中的7个氨基酸基序(残基123-129)需要通过介导病毒双链DNA (dsDNA)复制中间体的合成来招募NbPOLA2促进TbCSV的复制。这些发现有助于我们理解C3蛋白增强TbCSV复制的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication.

The C3 protein of tobacco curly shoot virus (TbCSV), a possible evolutionary intermediate between truly monopartite begomoviruses and those requiring satellite molecules for infectivity, has been identified as a viral replication enhancer (REn). However, the mechanisms underlying this enhancement are largely unknown. In this study, we generated two mutant infectious clones of TbCSV: one with a deletion of the 3' end region of the C3 gene that does not overlap with C2 (TbCSVdC3) and another in which this region was replaced by a phylogenetically unrelated iLOV gene sequence (TbCSVdC3-iLOV). Our findings highlight the crucial role of the 3' end region of C3 for viral DNA accumulation and further demonstrated that overexpression of TbCSV C3 protein in trans complements the functional deficiency of TbCSVdC3. Further analyses revealed the essential role of the C-terminal seven-amino acid motif from residues 123-129 of C3 in replication enhancement. Previous studies suggested that both intra- and intermolecular interactions of C3/AC3 proteins encoded by some other geminiviruses are vital for their capacity to enhance replication. Interestingly, among the tested potential interactors, NbPOLA2, a subunit of DNA polymerase α, was confirmed to interact with C3 in yeast and in planta. Our findings indicated that NbPOLA2 positively regulates TbCSV replication and infection and that the seven-amino acid motif (residues 123-129) in C3 is required for recruiting NbPOLA2 to facilitate TbCSV replication by mediating the viral double-stranded DNA (dsDNA) replication intermediate synthesis. These findings contribute to our understanding of the mechanisms through which the C3 protein enhances TbCSV replication.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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