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IF 2.4 3区医学 Q2 ACOUSTICS

Ultrasound in Medicine and Biology Pub Date : 2025-03-01 DOI:10.1016/j.ultrasmedbio.2025.02.007

Xing Hu, Gaobo Zhang, Xiandi Zhang, Yong Wang, Rong Xie, Xin Liu, Dean Ta, Hong Ding

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引用次数: 0

摘要

目的：微循环异质性在胶质母细胞瘤（GBM）的发生和发展过程中起着至关重要的作用。本研究采用超分辨率超声成像技术来观察 GBM 中的微循环异质性，目的是说明其在组织学评估中的预测价值：这项体内研究使用 15 只 Sprague-Dawley 大鼠探索 GBM 模型中的微血管，根据肿瘤生长阶段（植入后 12、18 和 24 d）分为三组。采用超声定位显微镜（ULM）评估微血管形态、血流动力学和异质性。采用 Kruskal-Wallis H 检验或方差分析，然后进行 Bonferroni 校正，对不同肿瘤生长阶段的结构、功能和异质性参数进行量化，以确定肿瘤进展的特征。评估了这些定量参数与病理指标（包括组织学血管密度（VD-H）、增殖指数和组织学血管成熟指数（VMI-H））之间的线性相关性。建立了一个逐步线性回归模型，以评估与组织学参数相关的预测性能：结果：与组织学相比，超低密度脂蛋白胆固醇能更早地发现肿瘤的进展。与肿瘤大小和免疫组化等传统指标相比，ULM 得出的定量参数能提供更全面的评估。多变量分析显示，曲率、血流方向方差（OV）和 VD-H 之间存在明显的相关性。此外，曲率、血流和 OV 与增殖指数有显著相关性，而血流和分形维度与 VMI-H 有显著相关性。与微血管形态学和功能灌注相比，异质性参数对某些组织学特征的预测能力更强：ULM为大鼠GBM微血管结构的早期、无创活体成像和量化提供了基础，并展示了对组织学参数的超分辨率预测能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An Early Progression Biomarker in Glioblastoma: Microcirculatory Heterogeneity on Ultrasound Localization Microscopy.

Objective: Microcirculatory heterogeneity plays an essential role in the initiation and progression of glioblastoma (GBM). This study employs super-resolution ultrasound imaging to visualize the microcirculatory heterogeneity in GBM, with the objective of illustrating its predictive value in histological assessments.

Methods: This in vivo study explored the microvasculature in GBM models using 15 Sprague-Dawley rats, divided into three groups based on tumor growth stages (12, 18 and 24 d post-implantation). Ultrasound localization microscopy (ULM) was employed to assess microvascular morphology, hemodynamics and heterogeneity. Structural, functional and heterogeneity parameters at different tumor growth stages were quantified using Kruskal-Wallis H tests, or analysis of variance, followed by Bonferroni correction to characterize tumor progression. Linear correlations between these quantitative parameters and pathological indicators, including histological vascular density (VD-H), proliferation index and histological vascular maturity index (VMI-H), were evaluated. A stepwise linear regression model was constructed to assess the predictive performance in relation to histological parameters.

Results: Compared to histology, ULM enabled the earlier detection of tumor progression. The quantitative parameters derived from ULM provided a more comprehensive assessment than conventional metrics such as tumor size and immunohistochemistry. Multivariate analysis exhibited significant correlations among curvature, blood flow orientation variance (OV) and VD-H. Additionally, curvature, blood flow and OV demonstrated significant correlations with the proliferation index, while blood flow and fractal dimension showed significant associations with VMI-H. Heterogeneity parameters exhibited superior predictive power for certain histological features compared to microvascular morphology and functional perfusion.

Conclusion: ULM provides a basis for early, non-invasive in vivo imaging and quantification of microvascular structures in rat GBM and demonstrates super-resolution predictive capability for histological parameters.

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来源期刊

Ultrasound in Medicine and Biology 医学-核医学

CiteScore

6.20

自引率

6.90%

发文量

325

审稿时长

70 days

期刊介绍： Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.