阿片受体基因单核苷酸多态性对埃及人群阿片使用障碍易感性的影响：一项病例对照研究

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-02-28 DOI:10.1016/j.genrep.2025.102180

Amira A. Abdelnoor , Mostafa M. Kamel , Fatma M. Elgazzar , Afaf M. Elsaid , Anas M. AboSamak

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引用次数: 0

摘要

背景：类鸦片样物质使用障碍（OUD）的易感性、进展和过程是由生物、发育、环境和遗传因素驱动的。阿片受体（OPR）基因的单核苷酸多态性（snp）可以影响受体的表达、结构或功能，可能改变OUD的易感性，影响治疗反应和复发率。Delta受体1 （OPRD1）、kappa受体1 （OPRK1）和mu受体1 （OPRM1）是研究较多的oud相关基因。他们在不同的种族群体中进行了测试，结果相互矛盾。因此，本研究旨在确定埃及人样本中社会人口统计学和遗传因素对OUD风险的影响。它填补了理解OPR基因内snp对埃及人OUD影响的关键空白。方法本病例对照研究评估了50名阿片类药物使用者与50名年龄和性别匹配的健康非药物使用者。从医疗记录和半结构化访谈中收集社会人口概况和阿片类药物使用数据。通过DSM-5和ICD-11症状检查表对参与者进行评估。采用OPRD1基因T921C、OPRK1基因G36T、OPRM1基因A118G的snp。采集静脉血进行DNA提取，在紫外透照器下进行PCR扩增，检测基因snp。结果OUD组在OPRD1（2%）、OPRK1（10%）和OPRM1（2%）中表现出多态性，snp与OUD之间无显著相关性。多变量回归分析确定了重要的OUD危险因素，包括低教育水平和积极的SU家族史，它们与OUD的可能性增加分别为8倍和6倍。结论本研究提供了初步证据，表明埃及人的OUD易感性主要与社会人口因素有关，而与OPR基因的遗传多态性无关。大学前教育（包括文盲）和接受过中小学教育的参与者，OUD易感风险增加了8倍(P值<；0.001，校正优势比8.652,95%置信区间)。阳性的SU家族史与易感性增加相关超过6倍（P值0.029，校正优势比6.101,95%置信区间）。

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The influence of single-nucleotide polymorphisms in opioid receptors genes on opioid use disorder susceptibility among Egyptian population: A case-control study

Background

Opioid use disorder (OUD) vulnerability, progression, and course are driven by biological, developmental, environmental, and genetic factors. Single nucleotide polymorphisms (SNPs) in opioid receptor (OPR) genes can influence receptor expression, structure, or function, potentially altering OUD susceptibility and impacting treatment response and relapse rates. Delta receptors 1 (OPRD1), kappa receptors 1 (OPRK1), and mu receptors 1 (OPRM1) are commonly studied OUD-related genes. They were examined in different ethnic groups, and their results conflicted. Therefore, this research aimed to determine the impact of sociodemographic and genetic factors on OUD risk in a sample of Egyptians. It fills a crucial gap in understanding the effect of SNPs within OPR genes on OUD among Egyptians.

Methods

This case-control study evaluated 50 opioid substance users versus 50 healthy, age- and sex-matched non-substance users. The sociodemographic profiles and opioid use data were collected from medical records and semi-structured interviews. Participants were assessed through the DSM-5 and ICD-11 Symptom Checklist. The SNPs in T921C of OPRD1, G36T of OPRK1, and A118G of OPRM1 genes were adopted. Venous blood samples were collected for DNA extraction and gene SNPs were examined after PCR amplification under an ultraviolet transilluminator.

Results

The OUD group exhibited polymorphisms in OPRD1 (2 %), OPRK1 (10 %), and OPRM1 (2 %) with no significant associations between SNPs and OUD. Multivariable regression analysis identified important OUD risk factors, including low education levels and a positive family history of SU. They were associated with an increased likelihood of OUD with 8 and 6 times, respectively.

Conclusion

This study provides initial evidence suggesting that OUD susceptibility among Egyptians is mainly related to sociodemographic factors rather than genetic polymorphisms in OPR genes. Pre-university education, including illiterate participants, as well as participants with primary and secondary education, increased OUD susceptibility risk >8 times (P value < 0.001, adjusted odds ratio 8.652 with 95 % confidence interval). A positive family history of SU was linked with increased susceptibility by more than sixfold (P value 0.029, adjusted odds ratio 6.101 with 95 % confidence interval).

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.