Suelen C. Lima, André C. de Oliveira, Maria Luiza L. da Costa, Dayane D. Abensur, Aylane Tamara dos S. Andrade, Hergen V. de Souza, Cláudia P. S. Tavares, Sergio M. Nunomura, Rita C. S. Nunomura, Rosemary A. Roque
{"title":"短叶红挥发油及主要化合物对埃及伊蚊幼虫的杀幼虫效果及作用机理及其对非目标水生动物的保护作用","authors":"Suelen C. Lima, André C. de Oliveira, Maria Luiza L. da Costa, Dayane D. Abensur, Aylane Tamara dos S. Andrade, Hergen V. de Souza, Cláudia P. S. Tavares, Sergio M. Nunomura, Rita C. S. Nunomura, Rosemary A. Roque","doi":"10.1007/s10340-024-01861-3","DOIUrl":null,"url":null,"abstract":"<p>Botanical larvicides, such as essential oils (EO) from the <i>Piper</i> species, offer eco-friendly mosquito control by targeting Culicidae larvae while exhibiting low toxicity to non-target aquatic animals. This study investigated the larvicidal activity and mechanism of action of the essential oil (EO) from <i>Piper brachypetiolatum</i> and its main compound, (<u>E</u>)-nerolidol, against <i>Aedes aegypti</i>, as well as the effects on non-target aquatic organisms <i>Toxorhynchites haemorrhoidalis</i>, <i>Anisops bouvieri</i>, and <i>Diplonychus indicus</i>. The EO was extracted from the leaves of <i>P. brachypetiolatum</i> using hydrodistillation, yielding 1.5 ± 0.7%. Gas chromatography revealed the presence of sesquiterpenes (64.70%), oxygenated sesquiterpenes (17.64%), monoterpenes (11.76%), and oxygenated monoterpenes (5.88%), with (<i>E</i>)-nerolidol as the major compound (64.32%). The EO and (<i>E</i>)-nerolidol exhibited significant larvicidal activity against A<i>. aegypti</i>, with LC<sub>50</sub> values of 15.51 and 9.50 mg/L, respectively. They also inhibited AChE activity (IC<sub>50</sub> values of 44.97 and 11.07 mg/L, respectively) and induced RONS overproduction (<i>p</i> < 0.05) compared to the positive control, α-cypermethrin. Additionally, the EO and (<i>E</i>)-nerolidol showed no lethal effects on <i>T. haemorrhoidalis</i>, <i>A. bouvieri</i>, and <i>D. indicus</i>, with these species exhibiting 100% survival after exposure. In contrast, <i>α</i>-cypermethrin caused 100% mortality in these species. These findings highlight the potential of the EO from <i>P. brachypetiolatum</i> and (<i>E</i>)-nerolidol as effective and environmentally friendly alternatives for controlling <i>A. aegypti</i> larvae.</p>","PeriodicalId":16736,"journal":{"name":"Journal of Pest Science","volume":"28 1","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Larvicidal effect and mechanism of action of the essential oil and major compound from Piper brachypetiolatum against Aedes aegypti larvae, with protection of non-target aquatic animals\",\"authors\":\"Suelen C. Lima, André C. de Oliveira, Maria Luiza L. da Costa, Dayane D. Abensur, Aylane Tamara dos S. Andrade, Hergen V. de Souza, Cláudia P. S. Tavares, Sergio M. Nunomura, Rita C. S. Nunomura, Rosemary A. Roque\",\"doi\":\"10.1007/s10340-024-01861-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Botanical larvicides, such as essential oils (EO) from the <i>Piper</i> species, offer eco-friendly mosquito control by targeting Culicidae larvae while exhibiting low toxicity to non-target aquatic animals. This study investigated the larvicidal activity and mechanism of action of the essential oil (EO) from <i>Piper brachypetiolatum</i> and its main compound, (<u>E</u>)-nerolidol, against <i>Aedes aegypti</i>, as well as the effects on non-target aquatic organisms <i>Toxorhynchites haemorrhoidalis</i>, <i>Anisops bouvieri</i>, and <i>Diplonychus indicus</i>. The EO was extracted from the leaves of <i>P. brachypetiolatum</i> using hydrodistillation, yielding 1.5 ± 0.7%. Gas chromatography revealed the presence of sesquiterpenes (64.70%), oxygenated sesquiterpenes (17.64%), monoterpenes (11.76%), and oxygenated monoterpenes (5.88%), with (<i>E</i>)-nerolidol as the major compound (64.32%). The EO and (<i>E</i>)-nerolidol exhibited significant larvicidal activity against A<i>. aegypti</i>, with LC<sub>50</sub> values of 15.51 and 9.50 mg/L, respectively. They also inhibited AChE activity (IC<sub>50</sub> values of 44.97 and 11.07 mg/L, respectively) and induced RONS overproduction (<i>p</i> < 0.05) compared to the positive control, α-cypermethrin. Additionally, the EO and (<i>E</i>)-nerolidol showed no lethal effects on <i>T. haemorrhoidalis</i>, <i>A. bouvieri</i>, and <i>D. indicus</i>, with these species exhibiting 100% survival after exposure. In contrast, <i>α</i>-cypermethrin caused 100% mortality in these species. 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Larvicidal effect and mechanism of action of the essential oil and major compound from Piper brachypetiolatum against Aedes aegypti larvae, with protection of non-target aquatic animals
Botanical larvicides, such as essential oils (EO) from the Piper species, offer eco-friendly mosquito control by targeting Culicidae larvae while exhibiting low toxicity to non-target aquatic animals. This study investigated the larvicidal activity and mechanism of action of the essential oil (EO) from Piper brachypetiolatum and its main compound, (E)-nerolidol, against Aedes aegypti, as well as the effects on non-target aquatic organisms Toxorhynchites haemorrhoidalis, Anisops bouvieri, and Diplonychus indicus. The EO was extracted from the leaves of P. brachypetiolatum using hydrodistillation, yielding 1.5 ± 0.7%. Gas chromatography revealed the presence of sesquiterpenes (64.70%), oxygenated sesquiterpenes (17.64%), monoterpenes (11.76%), and oxygenated monoterpenes (5.88%), with (E)-nerolidol as the major compound (64.32%). The EO and (E)-nerolidol exhibited significant larvicidal activity against A. aegypti, with LC50 values of 15.51 and 9.50 mg/L, respectively. They also inhibited AChE activity (IC50 values of 44.97 and 11.07 mg/L, respectively) and induced RONS overproduction (p < 0.05) compared to the positive control, α-cypermethrin. Additionally, the EO and (E)-nerolidol showed no lethal effects on T. haemorrhoidalis, A. bouvieri, and D. indicus, with these species exhibiting 100% survival after exposure. In contrast, α-cypermethrin caused 100% mortality in these species. These findings highlight the potential of the EO from P. brachypetiolatum and (E)-nerolidol as effective and environmentally friendly alternatives for controlling A. aegypti larvae.
期刊介绍:
Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues.
Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates.
Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management.
Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.