通过 DNA 稳定同位素探测揭开海洋塑料降解器的神秘面纱

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-03-01 DOI:10.1016/j.jhazmat.2025.137729

C Odobel, J Jacquin, C Saint Picq, M Hingant, P Lemechko, T Thomas, Boris Eyheraguibel, A Cébron, A ter Halle, , S Bruzaud, JF Ghiglione

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引用次数: 0

摘要

在自然环境中，塑料的生物降解是由生活在塑料表面的微生物生物膜来完成的。本研究首次利用稳定同位素示踪剂鉴定了海洋环境中的塑料降解物。在90天内，通过监测微生物细胞生长（通过扫描电子显微镜和流式细胞术）和活性（通过连续耗氧量测量和用于蛋白质合成的3H-leucine掺入），证明了聚羟基丁酸（PHB）的生物降解。我们成功合成了13c标记的PHB，并在不同的时间点进行了dna稳定同位素探测（DNA-SIP）实验，首次描述了PHB的主要降解物，包括Marinobacter sp.和Cellvibrionaceae，以及Glaciecola sp.、Pseudoalteromonas sp.、Celeribacter sp.和Alteromonas sp.。SIP标记结合元条形码被证明是发现和表征复杂海洋群落中活性塑料降解物的有用工具。

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Unveiling marine plastic degraders through DNA-stable isotope probing

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Unveiling marine plastic degraders through DNA-stable isotope probing

Plastic biodegradation in natural environments is performed by the microbial biofilm living on its surface. This study identifies for the first time plastic degraders in marine environment, by using stable isotope tracers. Polyhydroxybutyrate (PHB) biodegradation was proved by monitoring microbial cell growth (via scanning electron microscopy and flow cytometry) and activities (via continuous oxygen consumption measurements and ³H-leucine incorporation for protein synthesis) during 90 days. We successfully synthetized ¹³C-labeled PHB and conducted DNA-stable isotope probing (DNA-SIP) experiments over different time points, which permitted the first description of key PHB degraders belonging to Marinobacter sp. and Cellvibrionaceae together with Glaciecola sp., Pseudoalteromonas sp., Celeribacter sp. and Alteromonas sp. Overall, SIP labeling combined with metabarcoding proved to be a useful tool for discovering and characterizing active plastic degraders from complex marine communities.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.