基于具有过氧化物酶模拟活性和荧光特性的超薄 Fe-MOF-74 纳米片的用于急性心肌梗死早期诊断的新型荧光和比色双模式传感器

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-02-28 DOI:10.1016/j.aca.2025.343800

Jinyan Liu , Yong Wang , Weijie Peng , Bin Qiu , Kwok-yin Wong , Shuisheng Hu

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摘要

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties

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A novel fluorometric and colorimetric dual-mode sensor for AMI early diagnosis based on an ultrathin Fe-MOF-74 nanosheet with peroxidase mimic activity and fluorescence properties

Background

Cardiac troponin I (cTnI) is a crucial diagnosis biomarker for acute myocardial infarction (AMI). Early accurate determination of the concentration of cTnI significantly decreases the death rate of AMI. Compared with classic methods, dual-mode sensors for cTnI determination could help reduce the false positive rate.

Result

In this work, an ultrathin Fe-MOF-74 nanosheet with fluorescence properties and peroxidase mimic activity was synthesized for the first time. Applying this nanosheet, a novel dual-mode sensor was developed to quantify cTnI in human serum. The ultrathin Fe-MOF-74 nanosheets catalyze the decomposition of hydrogen peroxide produced by glucose oxidase (GOx)-triggered enzyme-linked immunosorbent assay (ELISA) applying cTnI as an antigen target, to generate reactive oxygen species (ROS). 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized by the generated free radicals, which simultaneously lead to the fluorescence quench of Fe-MOF-74 due to the inner filter effect (IFE). The correlation between the morphology of Fe-MOF-74 and its fluorescence intensity and peroxidase mimic activity was also investigated.

Significance

The sensors exhibited linearity with the concentration of cTnI in 10–2000 pg mL⁻¹ in both the fluorescence and visual mode with the detection limit of 6.4 pg mL⁻¹ and 8.4 pg mL⁻¹, respectively. It presenting good selectivity and anti-interference ability, can provide accurate and precise results in testing the concentration of cTnI in serum samples from patients in hospitals. It could be applied in the early diagnosis of AMI to reduce the incidence, disability, and mortality rates.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.