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IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-02-25 DOI:10.1016/j.plantsci.2025.112450

Kaixuan Yu , Yaxiao Song , Xiaohu Gao , Yingying Yang , Liya Huang , Jianghong Fu , Huijuan Yang , Xiaofei Wang , Yazhou Yang

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引用次数: 0

摘要

MdMYB1 基因在苹果花青素合成中起着关键作用，在着色初期可观察到截短的 MdMYB1 转录本。然而，MdMYB1 的异常转录及其在果实着色中的作用的机制仍不清楚。通过 RNA-Seq 和 qRT-PCR 分析，确定了两种转录形式，即 MdMYB1-S 和 MdMYB1-L。无功能的 MdMYB1-S 在着色初期高表达，而 MdMYB1-L 则主要在着色后期表达。3' RACE证实，MdMYB1-S转录本在第2内含子中以poly(A)终止。此外，内含子-2nd 中的三个转座元件（TEs）被高度甲基化，该内含子中的多聚腺苷酸化信号区在果实发育过程中甲基化程度不断增加。我们推测，内含子替代多腺苷酸化（APA）与DNA甲基化相结合，调节了MdMYB1-S向MdMYB1-L的转变，从而促进了果实着色。

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Intronic alternative polyadenylation in MdMYB1 regulates fruit coloration in apple

MdMYB1 gene plays a crucial role in anthocyanin synthesis in apple, with truncated MdMYB1 transcripts observed in the initial stage of coloration. However, the mechanism underlying the abnormal transcription of MdMYB1 and its role in fruit coloration remains unclear. Two transcript forms, MdMYB1-S and MdMYB1-L were identified through RNA-Seq and qRT-PCR analysis. The non-functional MdMYB1-S exhibited high expression in the initial coloration stage, while MdMYB1-L was predominantly expressed in the late coloration stage. 3′ RACE confirmed that MdMYB1-S transcripts terminated with poly(A) in intron-2nd. Additionally, three transposable elements (TEs) in intron-2nd were highly methylated, and the polyadenylation signal region in this intron demonstrated increasing methylation during fruit development. We hypothesize that intronic alternative polyadenylation (APA) in conjunction with DNA methylation, regulates the transition from MdMYB1-S to MdMYB1-L, thereby promoting fruit coloration.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.