{"title":"先天性巨细胞病毒感染小鼠模型中听觉而非前庭功能缺损的优势。","authors":"Chi-Chieh Chang, Ying-Chang Lu, Po-Nien Tsao, Yen-Hui Chan, Yi-Shan Lee, Pei-Hsuan Lin, Cheng-Yu Hsieh, Wei-Chung Hsu, Chuan-Jen Hsu, Chun-Yi Lu, Chen-Chi Wu","doi":"10.21053/ceo.2024.00305","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to investigate the auditory and vestibular consequences of congenital cytomegalovirus (cCMV) infection in a C57BL/6 mouse model established by intraperitoneal inoculation and designed to mimic clinical phenotypes.</p><p><strong>Methods: </strong>A cCMV infection model was established by intraperitoneal inoculation of murine cytomegalovirus into C57BL/6 mice. Auditory and vestibular function were assessed by auditory brainstem response, open field, swim, and rotarod tests. Histologic evaluation of the inner ear was also performed.</p><p><strong>Results: </strong>The study successfully established a model of cCMV infection in C57BL/6 mice. Auditory deficits of varying severity and laterality were observed, reflecting the clinical spectrum in humans. Vestibular function was minimally affected and did not correlate with auditory deficits. Histopathologic examination revealed predominant damage to spiral ganglion neurons with relatively intact vestibular organs.</p><p><strong>Conclusion: </strong>Our optimized mouse model effectively recapitulates the inner ear manifestations of human cCMV infection. The predominance of auditory over vestibular deficits provides valuable insights for the development of targeted therapeutic interventions for inner ear sequelae of cCMV infection.</p>","PeriodicalId":10318,"journal":{"name":"Clinical and Experimental Otorhinolaryngology","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Predominance of Auditory but not Vestibular Deficits in the Mouse Model of Congenital Cytomegalovirus Infection.\",\"authors\":\"Chi-Chieh Chang, Ying-Chang Lu, Po-Nien Tsao, Yen-Hui Chan, Yi-Shan Lee, Pei-Hsuan Lin, Cheng-Yu Hsieh, Wei-Chung Hsu, Chuan-Jen Hsu, Chun-Yi Lu, Chen-Chi Wu\",\"doi\":\"10.21053/ceo.2024.00305\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The aim of this study was to investigate the auditory and vestibular consequences of congenital cytomegalovirus (cCMV) infection in a C57BL/6 mouse model established by intraperitoneal inoculation and designed to mimic clinical phenotypes.</p><p><strong>Methods: </strong>A cCMV infection model was established by intraperitoneal inoculation of murine cytomegalovirus into C57BL/6 mice. Auditory and vestibular function were assessed by auditory brainstem response, open field, swim, and rotarod tests. Histologic evaluation of the inner ear was also performed.</p><p><strong>Results: </strong>The study successfully established a model of cCMV infection in C57BL/6 mice. Auditory deficits of varying severity and laterality were observed, reflecting the clinical spectrum in humans. Vestibular function was minimally affected and did not correlate with auditory deficits. Histopathologic examination revealed predominant damage to spiral ganglion neurons with relatively intact vestibular organs.</p><p><strong>Conclusion: </strong>Our optimized mouse model effectively recapitulates the inner ear manifestations of human cCMV infection. The predominance of auditory over vestibular deficits provides valuable insights for the development of targeted therapeutic interventions for inner ear sequelae of cCMV infection.</p>\",\"PeriodicalId\":10318,\"journal\":{\"name\":\"Clinical and Experimental Otorhinolaryngology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and Experimental Otorhinolaryngology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21053/ceo.2024.00305\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"OTORHINOLARYNGOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Otorhinolaryngology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21053/ceo.2024.00305","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
Predominance of Auditory but not Vestibular Deficits in the Mouse Model of Congenital Cytomegalovirus Infection.
Objective: The aim of this study was to investigate the auditory and vestibular consequences of congenital cytomegalovirus (cCMV) infection in a C57BL/6 mouse model established by intraperitoneal inoculation and designed to mimic clinical phenotypes.
Methods: A cCMV infection model was established by intraperitoneal inoculation of murine cytomegalovirus into C57BL/6 mice. Auditory and vestibular function were assessed by auditory brainstem response, open field, swim, and rotarod tests. Histologic evaluation of the inner ear was also performed.
Results: The study successfully established a model of cCMV infection in C57BL/6 mice. Auditory deficits of varying severity and laterality were observed, reflecting the clinical spectrum in humans. Vestibular function was minimally affected and did not correlate with auditory deficits. Histopathologic examination revealed predominant damage to spiral ganglion neurons with relatively intact vestibular organs.
Conclusion: Our optimized mouse model effectively recapitulates the inner ear manifestations of human cCMV infection. The predominance of auditory over vestibular deficits provides valuable insights for the development of targeted therapeutic interventions for inner ear sequelae of cCMV infection.
期刊介绍：
Clinical and Experimental Otorhinolaryngology (Clin Exp Otorhinolaryngol, CEO) is an international peer-reviewed journal on recent developments in diagnosis and treatment of otorhinolaryngology-head and neck surgery and dedicated to the advancement of patient care in ear, nose, throat, head, and neck disorders. This journal publishes original articles relating to both clinical and basic researches, reviews, and clinical trials, encompassing the whole topics of otorhinolaryngology-head and neck surgery.
CEO was first issued in 2008 and this journal is published in English four times (the last day of February, May, August, and November) per year by the Korean Society of Otorhinolaryngology-Head and Neck Surgery. The Journal aims at publishing evidence-based, scientifically written articles from different disciplines of otorhinolaryngology field.
The readership contains clinical/basic research into current practice in otorhinolaryngology, audiology, speech pathology, head and neck oncology, plastic and reconstructive surgery. The readers are otolaryngologists, head and neck surgeons and oncologists, audiologists, and speech pathologists.
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