成人哮喘的性别差异——let-7家族microrna的潜在免疫调节作用

IF 4.6 2区 医学 Q2 ALLERGY
Carina Malmhäll, Jenny Calvén, Julie Weidner, Kristina Johansson, Patricia Ramos-Ramírez, Emma Boberg, Linda Ekerljung, Roxana Mincheva, Bright Nwaru, Hannu Kankaanranta, Henric Olsson, Christopher McCrae, Madeleine Rådinger
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引用次数: 0

摘要

研究背景:在哮喘的发病率、患病率和严重程度上存在性别差异。先前动物模型的研究结果表明,炎症途径的性别差异可能导致哮喘发病,但人类研究有限。方法对成年哮喘患者(男、女)和健康人(男、女)分别采集气道和血液标本55份和85份。流式细胞术检测2型先天淋巴样细胞(ILC2s)、辅助性T细胞(Th)2及其IL-33R/ST2 (ST2L)的表达。ELISA法检测IL-13、胸腺基质淋巴生成素(TSLP)、IL-33和可溶性IL-33R/ST2 (sST2)。采用qPCR检测支气管活检组织中Let-7 miRNA的表达。结果与男性哮喘患者相比,女性哮喘患者有更多的急性发作和气道嗜酸性粒细胞数量。支气管活检中Let-7f、Let-7g和miR-98的表达在男性哮喘患者中高于女性,且与哮喘加重呈负相关。与男性相比,女性哮喘患者IL-13、TSLP和sST2水平升高。结论我们的研究表明,男性和女性哮喘患者的炎症特征不同。Let-7 mirna通过抑制IL-13的产生作为免疫调节剂,可能是解释哮喘性别差异的一个重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sex disparity in adult asthma—A potential immunomodulatory role of let-7 family microRNAs

Sex disparity in adult asthma—A potential immunomodulatory role of let-7 family microRNAs

Background

Sex differences have been reported in the incidence, prevalence and severity of asthma. Previous findings from animal models have revealed sex-related differences in inflammatory pathways that may contribute to asthma pathogenesis, but human studies are limited.

Methods

Airway and blood samples (n = 55 and n = 85 respectively) were collected from adult females and males with asthma and healthy subjects. Type 2 innate lymphoid cells (ILC2s), T helper (Th)2 cells and their expression of IL-33R/ST2 (ST2L) were evaluated by flow cytometry. IL-13, thymic stromal lymphopoietin (TSLP), IL-33 and soluble IL-33R/ST2 (sST2) were measured by ELISA. Let-7 miRNA expression in bronchial biopsies was determined by qPCR.

Results

Females with asthma reported more exacerbations and had a higher number of airway eosinophils compared with males with asthma. Bronchial biopsy expression of Let-7f, Let-7g and miR-98 tended to be higher in males with asthma compared with females and inversely correlated with asthma exacerbations. In contrast, increased levels of IL-13, TSLP and sST2 were found in females with asthma compared with males.

Conclusion

Our study demonstrates different inflammatory signatures between males and females with asthma. Let-7 miRNAs act as immune modulators by inhibiting the production of IL-13 and may be an important factor explaining the sex disparity seen in asthma.

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来源期刊
Clinical and Translational Allergy
Clinical and Translational Allergy Immunology and Microbiology-Immunology
CiteScore
7.50
自引率
4.50%
发文量
117
审稿时长
12 weeks
期刊介绍: Clinical and Translational Allergy, one of several journals in the portfolio of the European Academy of Allergy and Clinical Immunology, provides a platform for the dissemination of allergy research and reviews, as well as EAACI position papers, task force reports and guidelines, amongst an international scientific audience. Clinical and Translational Allergy accepts clinical and translational research in the following areas and other related topics: asthma, rhinitis, rhinosinusitis, drug hypersensitivity, allergic conjunctivitis, allergic skin diseases, atopic eczema, urticaria, angioedema, venom hypersensitivity, anaphylaxis, food allergy, immunotherapy, immune modulators and biologics, animal models of allergic disease, immune mechanisms, or any other topic related to allergic disease.
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