ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC双报告系的建立用于胰腺分化监测

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-15 DOI:10.1016/j.scr.2025.103685

Eunike Sawitning Ayu Setyono , Nicole Katarina Rogers , Anita Hofmann , Heiko Lickert , Ingo Burtscher

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引用次数: 0

摘要

胰岛由几种不同的内分泌细胞组成，它们和谐地工作。除原代胰岛外，干细胞衍生胰岛可作为替代研究和疾病模型。在此，我们在先前发表的ARX-CFP hiPSC系中通过CRISPR/ cas9介导的mCherry插入c肽的c端，引入了ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC的双报告系。该报告线允许在整个分化过程中实时监测干细胞衍生的胰腺α和β细胞。

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Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.