靶向超声增强成像定量子痫前期大鼠胎盘分子变化。

IF 3 4区 医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Molecular Imaging and Biology Pub Date : 2025-04-01 Epub Date: 2025-02-27 DOI:10.1007/s11307-025-01988-4
Lili Shi, Allan K N Alencar, Kenneth F Swan, Dylan J Lawrence, Gabriella Pridjian, Carolyn L Bayer
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引用次数: 0

摘要

目的:胎盘重构异常与多种妊娠相关疾病有关,包括先兆子痫(PE)。本研究应用双室(BCM)模型量化胎盘分子表达变化,提示胎盘重构异常,并评价靶向超声造影(T-CEUS)检测胎盘血管异常的有效性。BCM模型通过分析造影剂的分布,提供了胎盘内解剖动脉结构的高时间分辨率和分化。方法:采用一种由充气微泡(MB)组成的靶向造影剂(TCA),结合靶向血管生成生物标志物ανβ3整合素的表面偶联肽,用于定量胎盘血管发育。对实验性子宫灌注压降低(RUPP)胎盘功能不全的妊娠大鼠进行超声造影。在妊娠21天的第18天,分别获得正常妊娠(NP;n = 6)和RUPP (n = 6)。BCM模型用于估计TCA的结合动力学,提供胎盘结合常数(K b)的参数图。结果:与NP组相比,RUPP组的K -b值显著降低(p - K -b与其他分析(差异靶增强,dTE和晚期增强,LE)相比,表明它可以区分解剖动脉结构,具有更高的对比背景比。结论:BCM方法可鉴别与PE相关的胎盘异常发育相关的分子变化。与dTE和LE相比,它还揭示了胎盘更复杂的内部解剖结构,提示BCM可以增强PE的早期发现和监测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying Molecular Changes in the Preeclamptic Rat Placenta with Targeted Contrast-Enhanced Ultrasound Imaging.

Purpose: Abnormal placental remodeling is linked to various pregnancy-related diseases, including preeclampsia (PE). This study applies a bicompartmental (BCM) model to quantify molecular expression changes in the placenta, indicative of abnormal placental remodeling, and evaluates the effectiveness of targeted contrast-enhanced ultrasound (T-CEUS) in detecting the abnormal placental vasculature. The BCM model provides high temporal resolution and differentiation of anatomical artery structures within the placenta by analyzing the distribution of contrast agents.

Methods: A targeted contrast agent (TCA) composed of gas-filled microbubbles (MB), with a surface-conjugated peptide to target ανβ3 integrin, a biomarker for angiogenesis, was used for quantifying placental vascular development. CEUS images were acquired from timed pregnant Sprague Dawley rats with experimentally-induced reduced uterine perfusion pressure (RUPP) placental insufficiency. On gestational day (GD) 18 of a 21-day gestation, CEUS images were acquired from both Normal pregnant (NP; n = 6) and RUPP (n = 6) dams after injection of the TCA. The BCM model was used to estimate the binding dynamics of the TCA, providing a parametric map of the binding constant ( K b ) of the placenta.

Results: The RUPP group showed a significant reduction in the value of K b compared to the NP group (p < 0.05). A histogram of the placental K b was compared to alternative analyses (differential target enhancement, dTE and late enhancement, LE) to demonstrate that it can differentiate between anatomical artery structures with a higher contrast-to-background ratio.

Conclusions: The BCM method differentiates molecular changes associated with the abnormal placental development associated with PE. It also reveals more intricate internal anatomical structures of the placenta in comparison to dTE and LE, suggesting that the BCM could enhance early detection and monitoring of PE.

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来源期刊
CiteScore
6.90
自引率
3.20%
发文量
95
审稿时长
3 months
期刊介绍: Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.
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