线粒体呼吸能力影响大西洋鲑鱼鳃组织再生。

IF 2.8 2区 生物学 Q2 BIOLOGY
Journal of Experimental Biology Pub Date : 2025-04-01 Epub Date: 2025-04-07 DOI:10.1242/jeb.249704
Ensiyeh Ghanizadeh-Kazerouni, Benjamin Negrete, Simon R M Jones, Mark D Fast, Colin J Brauner
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引用次数: 0

摘要

鱼鳃再生在种间和种内均有差异。后者可能与包括能量代谢能力在内的多种因素有关。本研究通过饲喂旨在调节线粒体效率的实验饲料,研究线粒体呼吸能力是否影响鳃再生的程度,以及再生组织中线粒体的特征。在对照和试验饲料中饲养的鱼,对第一鳃弓腹侧和背侧的一部分细丝进行50%的纤维切除。线粒体呼吸和柠檬酸合成酶活性(CSA)分别在切除后第0周和第20周在再生组织中测定。在切除后第20周(第20周)测量纤维再生程度。实验饲料降低了CSA和呼吸控制率(RCR),增加了第0周的质子泄漏,与标准饲料相比,组织再生减少了30%。虽然再生组织中的CSA在第20周增加,但与第0周相比,状态3呼吸,质子泄漏,复合体IV活性和RCR均有所下降,与饮食无关。总体而言,第0周时线粒体呼吸效率与随后的鳃组织再生程度呈正相关。在第20周,状态3呼吸和质子泄漏与组织再生呈正相关,而CSA呈负相关。我们的研究结果表明,线粒体呼吸能力至少可以部分解释组织再生的个体间差异,但线粒体在再生组织中的功能可能是有限的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mitochondrial respiration capacity impacts gill tissue regeneration in Atlantic salmon.

Gill regeneration in fish varies inter- and intra-specifically. The latter may be associated with myriad factors including capacity of energy metabolism. This study investigated whether mitochondrial respiration capacity influences the degree of gill regeneration and features of mitochondria in regenerated tissue by feeding fish an experimental diet aimed at modulating mitochondrial efficiency. Atlantic salmon reared on standard and experimental diet were subjected to 50% filament resection on a subset of filaments on the ventral and dorsal regions of the first gill arch. Mitochondrial respiration and citrate synthase activity (CSA) were measured in the resected tips of filaments (week-0) and then in the regenerated tissue at 20 weeks post-resection (week-20). The degree of filament regeneration was measured at week-20. The experimental diet reduced CSA and respiratory control ratio (RCR), and increased proton leak at week-0, which was associated with a 30% reduction in tissue regeneration compared with fish on standard diet. While CSA increased in the regenerated tissue of experimental diet fish, there was a decline in other metrics of mitochondrial respiration including state 3, proton leak and RCR irrespective of diet. Overall, mitochondrial respiration efficiency at week-0 was positively correlated with the degree of subsequent gill tissue regeneration. Additionally, state 3 respiration and proton leak at week-20 were positively correlated with tissue regeneration, whereas CSA exhibited a negative relationship. Our results indicate that the capacity of mitochondrial respiration may at least partially explain the inter-individual variation in tissue regeneration, but mitochondrial function in the regenerating tissue may be limited.

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来源期刊
CiteScore
5.50
自引率
10.70%
发文量
494
审稿时长
1 months
期刊介绍: Journal of Experimental Biology is the leading primary research journal in comparative physiology and publishes papers on the form and function of living organisms at all levels of biological organisation, from the molecular and subcellular to the integrated whole animal.
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