{"title":"野生苏门答腊猩猩肺炎克雷伯菌的耐药性和毒力分析。","authors":"Usamah Afiff, Rahmat Hidayat, Agustin Indrawati, Titiek Sunartatie, Aprilia Hardiati, Dordia Anindita Rotinsulu, Raden Iis Arifiantini, Deandarla Naoremisa, Nurhashunatil Mar'ah, Safika Safika","doi":"10.5455/javar.2024.k858","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Orangutans (<i>Pongo abelii</i>), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. <i>Klebsiella pneumoniae</i> (<i>K. pneumonia</i>) and other Gram-negative bacteria are present in the Indigenous flora of many mammals, including orangutans. This study aimed to investigate the antibiotic resistance and virulence profile of <i>K. pneumonia</i> isolated from wild Sumatran orangutans.</p><p><strong>Materials and methods: </strong>This study investigated 10 fecal samples from wild Sumatran orangutans from the Gunung Leuser National Park, Aceh, Indonesia. Biochemical and molecular identification of <i>K. pneumoniae</i> using the RNA polymerase subunit b gene and detection of virulence-associated genes. In addition, molecular detection of antibiotic resistance genes was performed to characterize the resistance mechanisms in the isolates.</p><p><strong>Results: </strong><i>K. pneumonia</i> was detected in 6 out of 10 fecal samples from wild Sumatran orangutans. The virulence genes <i>mrk</i>D and <i>ent</i>B were detected in all (100%) of the isolates, whereas wabG was identified in 83.33% of the strains. Antibiotic susceptibility testing against <i>K. pneumoniae</i> revealed that three isolates were susceptible to streptomycin (S) and nalidixic acid (NA), while all six isolates were susceptible to chloramphenicol and ciprofloxacin. One isolate demonstrated intermediate resistance to NA, while the remaining two exhibited intermediate resistance to S. Six isolates were resistant to ampicillin, tetracycline, and erythromycin, indicating multidrug resistance. Furthermore, antibiotic resistance genes were detected in the isolates with the following prevalence: <i>bla</i> <sub>TEM</sub> gene (six isolates; 100%), <i>bla</i> <sub>SHV</sub> (six isolates; 100%), <i>bla</i> <sub>CTX-M</sub> gene (four isolates; 66.67%), and <i>tet</i>A gene (four isolates; 66.67%).</p><p><strong>Conclusion: </strong>This study revealed the virulence and resistance profile of <i>K. pneumoniae</i> bacterium isolated from wild Sumatran orangutans, which is essential for formulating effective conservation and healthcare strategies.</p>","PeriodicalId":14892,"journal":{"name":"Journal of Advanced Veterinary and Animal Research","volume":"11 4","pages":"1066-1075"},"PeriodicalIF":1.5000,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855422/pdf/","citationCount":"0","resultStr":"{\"title\":\"Antibiotic resistance and virulence profile of <i>Klebsiella pneumoniae</i> isolated from wild Sumatran Orangutans (<i>Pongo abelii</i>).\",\"authors\":\"Usamah Afiff, Rahmat Hidayat, Agustin Indrawati, Titiek Sunartatie, Aprilia Hardiati, Dordia Anindita Rotinsulu, Raden Iis Arifiantini, Deandarla Naoremisa, Nurhashunatil Mar'ah, Safika Safika\",\"doi\":\"10.5455/javar.2024.k858\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Orangutans (<i>Pongo abelii</i>), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. <i>Klebsiella pneumoniae</i> (<i>K. pneumonia</i>) and other Gram-negative bacteria are present in the Indigenous flora of many mammals, including orangutans. This study aimed to investigate the antibiotic resistance and virulence profile of <i>K. pneumonia</i> isolated from wild Sumatran orangutans.</p><p><strong>Materials and methods: </strong>This study investigated 10 fecal samples from wild Sumatran orangutans from the Gunung Leuser National Park, Aceh, Indonesia. Biochemical and molecular identification of <i>K. pneumoniae</i> using the RNA polymerase subunit b gene and detection of virulence-associated genes. In addition, molecular detection of antibiotic resistance genes was performed to characterize the resistance mechanisms in the isolates.</p><p><strong>Results: </strong><i>K. pneumonia</i> was detected in 6 out of 10 fecal samples from wild Sumatran orangutans. The virulence genes <i>mrk</i>D and <i>ent</i>B were detected in all (100%) of the isolates, whereas wabG was identified in 83.33% of the strains. Antibiotic susceptibility testing against <i>K. pneumoniae</i> revealed that three isolates were susceptible to streptomycin (S) and nalidixic acid (NA), while all six isolates were susceptible to chloramphenicol and ciprofloxacin. One isolate demonstrated intermediate resistance to NA, while the remaining two exhibited intermediate resistance to S. Six isolates were resistant to ampicillin, tetracycline, and erythromycin, indicating multidrug resistance. Furthermore, antibiotic resistance genes were detected in the isolates with the following prevalence: <i>bla</i> <sub>TEM</sub> gene (six isolates; 100%), <i>bla</i> <sub>SHV</sub> (six isolates; 100%), <i>bla</i> <sub>CTX-M</sub> gene (four isolates; 66.67%), and <i>tet</i>A gene (four isolates; 66.67%).</p><p><strong>Conclusion: </strong>This study revealed the virulence and resistance profile of <i>K. pneumoniae</i> bacterium isolated from wild Sumatran orangutans, which is essential for formulating effective conservation and healthcare strategies.</p>\",\"PeriodicalId\":14892,\"journal\":{\"name\":\"Journal of Advanced Veterinary and Animal Research\",\"volume\":\"11 4\",\"pages\":\"1066-1075\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-12-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855422/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Veterinary and Animal Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/javar.2024.k858\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Veterinary and Animal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/javar.2024.k858","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
摘要
目的：猩猩（Pongo abelii）是印度尼西亚特有的灵长类动物，其特点是主要生活在树上。肺炎克雷伯菌（肺炎克雷伯菌）和其他革兰氏阴性菌存在于包括猩猩在内的许多哺乳动物的本土菌群中。本研究旨在研究野生苏门答腊猩猩肺炎克雷伯菌的抗生素耐药性和毒力谱。材料与方法：本研究调查了印度尼西亚亚齐省Gunung Leuser国家公园野生苏门答腊猩猩的10份粪便样本。利用RNA聚合酶亚基b基因对肺炎克雷伯菌进行生化和分子鉴定及毒力相关基因检测。此外，还进行了抗生素耐药基因的分子检测，以表征菌株的耐药机制。结果：10份野生苏门答腊猩猩粪便中有6份检出肺炎克雷伯菌。所有菌株（100%）检出mrkD和entB毒力基因，83.33%检出wabG毒力基因。肺炎克雷伯菌药敏试验结果显示，3株菌株对链霉素(S)和钠啶酸（NA）敏感，6株菌株对氯霉素和环丙沙星均敏感。1株对NA表现出中等耐药，其余2株对s表现出中等耐药。6株对氨苄西林、四环素和红霉素均有耐药，显示为多药耐药。此外，在分离株中检测到抗生素耐药基因，其流行率如下：bla TEM基因(6株；100%), bla SHV(6株；100%)， bla CTX-M基因(4株；66.67%)， tetA基因(4株；66.67%)。结论：本研究揭示了野生苏门答腊猩猩肺炎克雷伯菌的毒力和耐药特征，为制定有效的保护和卫生保健策略提供了依据。
Antibiotic resistance and virulence profile of Klebsiella pneumoniae isolated from wild Sumatran Orangutans (Pongo abelii).
Objective: Orangutans (Pongo abelii), as endemic primates of Indonesia, are characterized by a predominantly arboreal lifestyle. Klebsiella pneumoniae (K. pneumonia) and other Gram-negative bacteria are present in the Indigenous flora of many mammals, including orangutans. This study aimed to investigate the antibiotic resistance and virulence profile of K. pneumonia isolated from wild Sumatran orangutans.
Materials and methods: This study investigated 10 fecal samples from wild Sumatran orangutans from the Gunung Leuser National Park, Aceh, Indonesia. Biochemical and molecular identification of K. pneumoniae using the RNA polymerase subunit b gene and detection of virulence-associated genes. In addition, molecular detection of antibiotic resistance genes was performed to characterize the resistance mechanisms in the isolates.
Results: K. pneumonia was detected in 6 out of 10 fecal samples from wild Sumatran orangutans. The virulence genes mrkD and entB were detected in all (100%) of the isolates, whereas wabG was identified in 83.33% of the strains. Antibiotic susceptibility testing against K. pneumoniae revealed that three isolates were susceptible to streptomycin (S) and nalidixic acid (NA), while all six isolates were susceptible to chloramphenicol and ciprofloxacin. One isolate demonstrated intermediate resistance to NA, while the remaining two exhibited intermediate resistance to S. Six isolates were resistant to ampicillin, tetracycline, and erythromycin, indicating multidrug resistance. Furthermore, antibiotic resistance genes were detected in the isolates with the following prevalence: blaTEM gene (six isolates; 100%), blaSHV (six isolates; 100%), blaCTX-M gene (four isolates; 66.67%), and tetA gene (four isolates; 66.67%).
Conclusion: This study revealed the virulence and resistance profile of K. pneumoniae bacterium isolated from wild Sumatran orangutans, which is essential for formulating effective conservation and healthcare strategies.
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