结核分枝杆菌细胞分裂素结合蛋白位点的研究。

IF 2.7 3区 生物学 Q3 MICROBIOLOGY
Journal of Bacteriology Pub Date : 2025-03-20 Epub Date: 2025-02-27 DOI:10.1128/jb.00003-25
Jin Hee Yoo, Cristina Santarossa, Audrey Thomas, Damian Ekiert, K Heran Darwin
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引用次数: 0

摘要

细胞分裂素是一种基于腺嘌呤的激素,已经在植物中得到了很好的表征,但也可以由细菌产生,包括人类独有的病原体结核分枝杆菌。像植物一样,结核分枝杆菌使用细胞分裂素来调节基因表达。我们之前已经证实,细胞分裂素在结核分枝杆菌中的过度积累会导致细胞分裂素分解过程中产生的醛的积累。在植物中,称为细胞分裂素氧化酶的专用酶将细胞分裂素转化为腺嘌呤和各种醛。蛋白酶体降解缺陷结核分枝杆菌不能降解产生细胞分裂素的酶Log,它会积累几种细胞分裂素和至少一种细胞分裂素相关的醛,导致对一氧化氮和铜的敏感性增加。因此,我们假设结核分枝杆菌编码一种或多种细胞分裂素氧化酶,这种酶的破坏可能会恢复蛋白酶体缺陷菌株对一氧化氮和铜的抵抗力。通过同源性检索,我们鉴定出Rv3719是一个与植物细胞分裂素氧化酶高度相似的蛋白。然而,该基因的删除并没有恢复一氧化氮或铜抗性的降解缺陷突变体。相反,我们观察到当Rv3719从野生型或蛋白酶体缺陷菌株中删除时,铜敏感性增加。最后,我们对Rv3718c进行了表征,Rv3718c是一个编码于Rv3719附近的蛋白,并发现它以高特异性结合细胞分裂素。总的来说,这些数据支持细胞分裂素活性在结核分枝杆菌生理学中的作用仍有待进一步阐明。许多细菌种类编码产生细胞分裂素的酶,其功能几乎完全未知。这项工作为细菌细胞分裂素领域提供了新的知识,并进一步揭示了植物和原核生物之间细胞分裂素相关蛋白的保护。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of a cytokinin-binding protein locus in Mycobacterium tuberculosis.

Cytokinins are adenine-based hormones that have been well-characterized in plants but are also made by bacteria, including the human-exclusive pathogen Mycobacterium tuberculosis. Like plants, M. tuberculosis uses cytokinins to regulate gene expression. We previously established that cytokinin overaccumulation in M. tuberculosis results in a buildup of aldehydes produced during cytokinin breakdown. In plants, dedicated enzymes called cytokinin oxidases convert cytokinins into adenine and various aldehydes. Proteasome degradation-deficient M. tuberculosis, which cannot degrade the cytokinin-producing enzyme Log, accumulates several cytokinins and at least one cytokinin-associated aldehyde, resulting in increased sensitivity to nitric oxide and copper. We therefore hypothesized that M. tuberculosis encodes one or more cytokinin oxidases, and disruption of this enzyme might restore resistance to nitric oxide and copper in a proteasome-defective strain. Using a homology-based search, we identified Rv3719 as a protein with high similarity to a plant cytokinin oxidase. Deletion of this gene, however, did not restore nitric oxide or copper resistance to a degradation-defective mutant. Instead, we observed increased copper sensitivity when Rv3719 was deleted from either wild-type or proteasome-defective strains. Finally, we characterized Rv3718c, a protein encoded adjacent to Rv3719, and found that it bound a cytokinin with high specificity. Collectively, these data support a role for cytokinin activity in M. tuberculosis physiology that remains to be further elucidated.IMPORTANCENumerous bacterial species encode cytokinin-producing enzymes, the functions of which are almost completely unknown. This work contributes new knowledge to the cytokinin field for bacteria and reveals further conservation of cytokinin-associated proteins between plants and prokaryotes.

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来源期刊
Journal of Bacteriology
Journal of Bacteriology 生物-微生物学
CiteScore
6.10
自引率
9.40%
发文量
324
审稿时长
1.3 months
期刊介绍: The Journal of Bacteriology (JB) publishes research articles that probe fundamental processes in bacteria, archaea and their viruses, and the molecular mechanisms by which they interact with each other and with their hosts and their environments.
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