利用生物模拟器分析医院重症监护病房内的环境微生物组。

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS
BioTechniques Pub Date : 2025-02-01 Epub Date: 2025-02-26 DOI:10.1080/07366205.2025.2467550
Sunil Thomas, Kyle Bittinger, Lawrence L Livornese
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引用次数: 0

摘要

医院获得性感染(HAIs),也称为医院感染,是在医疗机构治疗期间感染的疾病,可导致严重或危及生命的并发症。艾滋病是由对标准抗生素有耐药性的微生物引起的。HAIs可导致严重的并发症、更长的住院时间和更高的死亡率,特别是在脆弱的患者中。在我们之前的研究中,我们展示了一个被称为“生物模拟器”的雕刻培养皿的能力,以诱导非粘附细胞和微生物组的粘附。本文探讨了使用生物模拟器来阐明重症监护病房(icu)内的微生物组组成在医院设置。将生物模拟器与营养丰富的细菌生长培养基一起置于icu中24 h,然后在好氧和厌氧条件下孵育3天。使用16S rRNA测序,我们分析了来自多个样本的ICU微生物组。我们的研究结果表明,ICU的微生物组与患者的微生物组密切相关,ICU中的微生物比对照条件下的微生物表现出更强的相互关系。生物模拟器和分析的结合使用为分析和理解医疗保健环境中微生物组的变化提供了一种有效的方法,特别是在高风险地区,如icu。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital.

Hospital-acquired infections (HAIs), also known as nosocomial infections, are illnesses contracted during treatment at a healthcare facility and can result in severe or life-threatening complications. HAIs are caused by microorganisms that exhibit resistance to standard antibiotics. HAIs can lead to severe complications, longer stays, and increased mortality, particularly in vulnerable patients. In our previous study, we demonstrated the ability of an engraved Petri dish, referred to as a "biosimulator," to induce adhesion of non-adherent cells and the microbiome. This paper explores the use of the biosimulator to elucidate the microbiome composition within intensive care units (ICUs) in a hospital setting. The biosimulator, with a nutrient-rich bacterial growth medium, was placed in ICUs for 24 h, then incubated for three days under aerobic and anaerobic conditions. Using 16S rRNA sequencing, we profiled the ICU microbiome from multiple samples. Our findings showed that ICU microbiomes closely mirrored those of patients, with microorganisms in the ICU exhibiting stronger interrelationships than in control conditions. The combined use of the biosimulator and profiling offers an effective approach for analyzing and understanding microbiome changes in healthcare settings, particularly in high-risk areas, such as ICUs.

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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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