衍生化-液相色谱-串联质谱法同时测定人血清中25(OH)D2、25(OH)D3和1α，25(OH)2D3。

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-02-24 DOI:10.1016/j.ab.2025.115821

Zi-qing Wang , Li-ping Hao , Zi-xuan Meng , Hao-ran Zhang , Wei-jun Kang , Lian-feng Ai

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引用次数: 0

摘要

建立并验证了同时定量人血清中25-羟基维生素D2， D3和1α，25-二羟维生素D3的LC-MS/MS方法。用乙腈沉淀200 uL血清中的蛋白质。离心后，代谢产物用（二乙酰氧基）苯（PIA）和4-（4-二甲氨基苯基）-1,2,4-三唑烷-3,5-二酮（DMAT）衍生化，然后用LC-MS/MS系统定量。三种物质的检出限（lod）分别为10、10、5 pg/mL，定量限（loq）分别为20、20、10 pg/mL。在特定浓度范围内，标准曲线的线性回归系数（R2）为>0.998。25(OH)D2加样回收率为94.36% ~ 102.34%,25(OH) D3加样回收率为92.43% ~ 103.41%,1α,25(OH)2D3加样回收率为88.98% ~ 94.36%。109名受试者（包括61名健康成年男性和59名健康成年女性）的平均血清水平分别为2.0±1.5 ng/ml （25(OH)D2）、16.4±6.1 ng/ml （25(OH)D3）和36.6±15.1 pg/ml （1α, 25(OH)2D3）。利用PIA和dat衍生化维生素D代谢物可用于同时快速测定人血清中25(OH) D2、25(OH) D3和1α，25(OH)2D3浓度。

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Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry

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Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry

An LC-MS/MS method was developed and validated for simultaneously quantifying 25-hydroxyvitamin D2, D3, and 1α,25-dihydroxyvitamin D3 in human serum. Protein in 200 μL serum was precipitated with acetonitrile. After centrifugation, the metabolites were derivatized using (diacetoxyiodo)benzene (PIA) and 4-(4-dimethylaminophenyl)-1,2,4-triazolidine-3,5-dione (DMAT) and then quantified by the LC-MS/MS system. The limits of detection (LODs) for the three substances were 10, 10, and 5 pg/mL, and the limits of quantification (LOQs) were 20, 20, and 10 pg/mL. The standard curves for these compounds showed linear regression coefficients (R2)>0.998 over specific concentration ranges. Recoveries were 94.36 %–102.34 % for 25(OH)D2, 92.43 %–103.41 % for 25(OH)D3, and 88.98 %–94.36 % for 1α,25(OH)2D3. The mean serum levels in 109 subjects (consisting of 61 healthy adult males and 59 healthy adult females) were 2.0 ± 1.5 ng/ml (25(OH)D2), 16.4 ± 6.1 ng/ml (25(OH)D3) and 36.6 ± 15.1 pg/ml (1α, 25(OH)2D3).Derivatization of vitamin D metabolites using PIA and DMAT is useful for rapidly determining the serum 25(OH) D2, 25(OH) D3 and 1α,25(OH)2D3 concentrations simultaneously in human serum.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.