泰国自然感染猫支原体感染的分子调查和特征

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-02-28 DOI:10.1007/s11686-025-01002-5

Kiattisak Pimpjong, Kanokwan Ratyotha, Thanvarat Rungruang, Napaporn Thongpriam, Kotchaphon Vaisusuk, Supawadee Piratae

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引用次数: 0

摘要

支原体是附着在红细胞表面的嗜血性寄生虫，在受感染的猫中引起溶血性贫血。这些病原体如不及时治疗可导致严重的临床后果，包括死亡。尽管它们很重要，但在泰国对支原体感染的分子研究仍然有限。本研究旨在确定猫中支原体感染的流行程度，确定所涉及的特定物种，并评估感染猫的血液学变化。此外，还分析了支原体物种的系统发育关系。方法在泰国沙末普拉干、乌隆他尼、猜雅福和摩哈萨拉卡等4个省的兽医医院采集149只猫的血液样本，进行常规检查、接种疫苗或患病，未发现感染的具体迹象。采用针对16S rRNA基因的PCR检测支原体，并进行DNA测序和系统发育分析进行物种鉴定。结果总感染率为17.45% (95% CI: 11.73 ~ 24.51)，其中血支原体（CMhm）占14.09%，血支原体（Mhf）占2.01%，兔支原体（CMt）占1.34%。对26个阳性PCR产物进行测序，21个样品中鉴定出CMhm， 3个样品中鉴定出Mhf， 2个样品中鉴定出CMt，共鉴定出7种不同的单倍型。本研究中鉴定的所有支原体物种与GenBank数据库中现有的支原体物种核苷酸相似性为99-100%。血液学分析显示，除血小板计数有统计学意义的显著下降外，大多数参数无显著变化（P < 0.01）。结论本研究提供了支原体的分子流行病学和遗传鉴定信息，有助于更好地了解泰国猫的血原体感染，有助于这些微生物的控制。

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Molecular Investigation and Characterization of Mycoplasma spp. infections in Naturally Infected Cats in Thailand

Purpose

Mycoplasma spp. are hemotropic parasites that attach to the surface of red blood cells, causing hemolytic anemia in infected cats. These pathogens can result in significant clinical consequences, including death if left untreated. Despite their importance, molecular studies on Mycoplasma infections in Thailand remain limited. This study aimed to determine the prevalence of Mycoplasma spp. infections in cats, identify specific species involved, and assess hematologic changes in infected cats. Additionally, phylogenetic relationships of Mycoplasma species were analyzed.

Methods

A total of 149 blood samples were collected from cats visiting veterinary hospitals in four provinces of Thailand including Samut Prakan, Udon Thani, Chaiyaphum, and Maha Sarakham for routine check-ups, vaccinations, or illness, without specific signs of infection. PCR targeting the 16S rRNA gene was used to detect Mycoplasma spp., followed by DNA sequencing and phylogenetic analysis for species identification.

Results

The overall prevalence of Mycoplasma spp. infection was 17.45% (95% CI: 11.73–24.51), comprising Candidatus Mycoplasma haemominutum (CMhm) (14.09%), Mycoplasma haemofelis (Mhf) (2.01%), and Candidatus Mycoplasma turicensis (CMt) (1.34%). Sequencing of 26 positive PCR products identified CMhm in 21 samples, Mhf in 3 samples, and CMt in 2 samples, revealing seven distinct haplotypes. All Mycoplasma species identified in this study showed 99–100% nucleotide similarity with those available in the GenBank database. Hematologic analysis revealed no significant changes in most parameters, except for a statistically significant decrease in platelet count (P < 0.01).

Conclusions

This study provides information on the molecular epidemiology and genetic identification of Mycoplasma spp., contributing to a better understanding of hemoplasma infections in Thai cats, which will aid in the control of these microorganisms.

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.