功能金属有机框架介导的g -四重DNA纳米结构用于改进自供电智能手机辅助双模生物传感

IF 10.5 1区 生物学 Q1 BIOPHYSICS
Peiyuan Li , Yashu Wei , Shiyu Liu , Jiawen Wu , Yeyu Wu , Jun Yan , Shaogang Liu , Xuecai Tan , Ke-Jing Huang
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引用次数: 0

摘要

主要疾病生物标志物的准确检测经常受到复杂环境因素及其固有的低表达水平的阻碍。为了克服这些挑战,研究人员开发了一种自供电、智能手机辅助的双模生物传感平台,利用功能性金属有机框架(MOFs)和g -四重体(G-4s) DNA纳米结构显著放大信号输出并提高检测精度。该创新系统利用金纳米粒子/锆基MOF/石墨炔复合材料(AuNPs/Zr-MOF/GDY)和自组装亚甲基蓝(MB)与g -四重体(G-4s) DNA纳米结构来检测地中海贫血基因CD122。检测到目标后,G-4s结构触发链位移和阳离子配位,随后MB吸附形成生物阴极。这种生物阴极促进电子转移到葡萄糖氧化酶功能化的阳极,驱动系统生成电子的氧化还原反应。MB/G-4s结构不仅可以放大CD122信号,还可以诱导成比例的颜色变化,用于电化学和比色双检测。集成了一个高输出功率电容器,进一步放大信号,提高灵敏度7.2倍。该平台提供了从0.0001到1000 pM的宽线性范围,并实现了18.9 aM(电化学)和23.9 aM(比色)(S/N = 3)的低检测限。这项工作提供了一个高度准确,敏感和便携式的检测平台,可在各种环境中进行快速分析,包括远程和具有挑战性的设置。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional metal organic framework mediated G-quadruplex DNA nanostructures for improved self-powered smartphone-assisted dual-mode biosensing
Accurate detection of major disease biomarkers is frequently hindered by complex environmental factors and their inherently low expression levels. To overcome these challenges, a self-powered, smartphone-assisted dual-mode biosensing platform has been developed, utilizing functional metal-organic frameworks (MOFs) and G-quadruplex (G-4s) DNA nanostructures to significantly amplify signal output and enhance detection accuracy. This innovative system utilizes gold nanoparticle/zirconium-based MOF/graphdiyne composites (AuNPs/Zr-MOF/GDY) and self-assembles methylene blue (MB) with G-quadruplex (G-4s) DNA nanostructures for detecting the thalassemia gene CD122. Upon target detection, the G-4s structure triggers strand displacement and cation coordination, followed by MB adsorption to form a biocathode. This biocathode facilitates electron transfer to a glucose oxidase-functionalized anode, driving redox reactions with system-generated electrons. The MB/G-4s structure not only amplifies the CD122 signal but also induces proportional color changes for dual electrochemical and colorimetric detection. A high-output-power capacitor is integrated to further amplify signals, enhancing sensitivity by 7.2 times. The platform offers a broad linear range from 0.0001 to 1000 pM and achieves low detection limits of 18.9 aM (electrochemical) and 23.9 aM (colorimetric) (S/N = 3). This work presents a highly accurate, sensitive, and portable detection platform for rapid analysis in various environments, including remote and challenging settings.
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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