多功能磁电和外泌体负载水凝胶通过远程无创电刺激促进脊髓损伤后神经系统恢复的神经元分化和免疫调节

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2025-02-28 DOI:10.1016/j.bioactmat.2025.02.034

Wubo Liu , Qiang Liu , Zeqin Li , Chunjia Zhang , Zehui Li , Han Ke , Xin Xu , Xiaoxin Wang , Huayong Du , Zuliyaer Talifu , Yunzhu Pan , Xiaoxiong Wang , Jingyun Mao , Feng Gao , Degang Yang , Yan Yu , Xinyu Liu , Jianjun Li

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引用次数: 0

摘要

干预神经干细胞（NSCs）的分化是治疗脊髓损伤（SCI）的一种非常有前途的方法。然而，损伤部位的NSCs往往存在低存活率和不受控制的分化。尽管电刺激在调节NSCs的命运和促进组织修复方面已被证明是有效的，然而，由于诸如侵入性和技术复杂性等挑战，传统的电刺激疗法未能得到广泛应用。为了克服这些限制，我们开发了一种仿生磁电水凝胶，结合Fe3O4@BaTiO3核壳纳米颗粒和人脐带间充质干细胞外泌体（HUMSC-Exos），围绕构建远程无创电刺激协同治疗SCI的概念。Fe3O4@BaTiO3被外周磁场激活产生电刺激，结合HUMSC-Exos的协同作用，显著缓解脊髓损伤相关的早期炎症反应，增强新生神经元和轴突的再生，为脊髓损伤后功能恢复创造有利条件。我们的研究结果表明，在脊髓损伤大鼠模型中应用这种磁外泌体水凝胶可导致实质性的功能恢复。这种创新的组合为脊髓损伤修复提供了一种有前景的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Multifunctional magneto-electric and exosome-loaded hydrogel enhances neuronal differentiation and immunoregulation through remote non-invasive electrical stimulation for neurological recovery after spinal cord injury

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Intervention in the differentiation of neural stem cells (NSCs) is emerging as a highly promising approach for the treatment of spinal cord injury (SCI). However, NSCs at the injury site often suffer from low survival and uncontrolled differentiation. Whereas electrical stimulation has proven effective in regulating the fate of NSCs and promoting tissue repair, however, conventional electrical stimulation therapy has failed to be widely applied due to challenges such as invasiveness and technical complexity. To overcome these limitations, we developed a biomimetic magneto-electric hydrogel incorporating Fe₃O₄@BaTiO₃ core-shell nanoparticles and human umbilical mesenchymal stem cell exosomes (HUMSC-Exos) around the concept of constructing remote noninvasive electrical stimulation for the synergistic treatment of SCI. The Fe₃O₄@BaTiO₃ is activated by the peripheral magnetic field to generate electrical stimulation, which, in conjunction with the synergistic effects of HUMSC-Exos, significantly alleviates the early inflammatory response associated with SCI and enhances the regeneration of newborn neurons and axons, thereby creating favorable conditions for functional recovery post-SCI. Our findings indicate that applying this magneto-exosome hydrogel in a rat model of SCI leads to substantial functional recovery. This innovative combination represents a promising therapeutic strategy for SCI repair.

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.