山东灵芝甾体及其乙酰胆碱酯酶抑制活性研究。

IF 2.1 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Steroids Pub Date : 2025-02-23 DOI:10.1016/j.steroids.2025.109574

Yaqin Huo , Shiyuan Ma , Xihan He , Yuxi Wang , Haisheng Yuan

{"title":"山东灵芝甾体及其乙酰胆碱酯酶抑制活性研究。","authors":"Yaqin Huo , Shiyuan Ma , Xihan He , Yuxi Wang , Haisheng Yuan","doi":"10.1016/j.steroids.2025.109574","DOIUrl":null,"url":null,"abstract":"<div><div>Investigation of the solid culture of <em>Ganoderma shandongense</em> led to the isolation of 15 compounds, including one new steroid (compound <strong>1</strong>) and fourteen known ones (compounds <strong>2</strong>–<strong>15</strong>). Their structures were determined via extensive the nuclear magnetic resonance (NMR) spectroscopic analyses and quantum chemical calculations. Compounds <strong>3</strong>, <strong>9</strong>, and <strong>14</strong> exhibited inhibitory activities against acetylcholinesterase (AChE), with half maximal inhibitory concentration (IC<sub>50</sub>) values of 29.4, 29.4 and 33.2 µM, respectively. Furthermore, molecular docking studies were undertaken to elucidate the interaction mechanisms between the compounds and the amino acid residues of AChE.</div></div>","PeriodicalId":21997,"journal":{"name":"Steroids","volume":"216 ","pages":"Article 109574"},"PeriodicalIF":2.1000,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Steroids from the mushroom Ganoderma shandongense and their AChE inhibitory activities\",\"authors\":\"Yaqin Huo , Shiyuan Ma , Xihan He , Yuxi Wang , Haisheng Yuan\",\"doi\":\"10.1016/j.steroids.2025.109574\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Investigation of the solid culture of <em>Ganoderma shandongense</em> led to the isolation of 15 compounds, including one new steroid (compound <strong>1</strong>) and fourteen known ones (compounds <strong>2</strong>–<strong>15</strong>). Their structures were determined via extensive the nuclear magnetic resonance (NMR) spectroscopic analyses and quantum chemical calculations. Compounds <strong>3</strong>, <strong>9</strong>, and <strong>14</strong> exhibited inhibitory activities against acetylcholinesterase (AChE), with half maximal inhibitory concentration (IC<sub>50</sub>) values of 29.4, 29.4 and 33.2 µM, respectively. Furthermore, molecular docking studies were undertaken to elucidate the interaction mechanisms between the compounds and the amino acid residues of AChE.</div></div>\",\"PeriodicalId\":21997,\"journal\":{\"name\":\"Steroids\",\"volume\":\"216 \",\"pages\":\"Article 109574\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-02-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Steroids\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039128X25000157\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Steroids","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039128X25000157","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

对山东灵芝的固体培养进行了研究，共分离到15个化合物，其中1个为新化合物（化合物1），14个为已知化合物（化合物2 ~ 15）。通过广泛的核磁共振光谱分析和量子化学计算确定了它们的结构。化合物3、9和14对乙酰胆碱酯酶（AChE）具有抑制活性，半数最大抑制浓度（IC50）分别为29.4、29.4和33.2 µM。此外，还进行了分子对接研究，以阐明化合物与乙酰胆碱酯氨基酸残基之间的相互作用机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Steroids from the mushroom Ganoderma shandongense and their AChE inhibitory activities

查看原文本刊更多论文

Steroids from the mushroom Ganoderma shandongense and their AChE inhibitory activities

Investigation of the solid culture of Ganoderma shandongense led to the isolation of 15 compounds, including one new steroid (compound 1) and fourteen known ones (compounds 2–15). Their structures were determined via extensive the nuclear magnetic resonance (NMR) spectroscopic analyses and quantum chemical calculations. Compounds 3, 9, and 14 exhibited inhibitory activities against acetylcholinesterase (AChE), with half maximal inhibitory concentration (IC₅₀) values of 29.4, 29.4 and 33.2 µM, respectively. Furthermore, molecular docking studies were undertaken to elucidate the interaction mechanisms between the compounds and the amino acid residues of AChE.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Steroids 医学-内分泌学与代谢

CiteScore

5.10

自引率

3.70%

发文量

120

审稿时长

73 days

期刊介绍： STEROIDS is an international research journal devoted to studies on all chemical and biological aspects of steroidal moieties. The journal focuses on both experimental and theoretical studies on the biology, chemistry, biosynthesis, metabolism, molecular biology, physiology and pharmacology of steroids and other molecules that target or regulate steroid receptors. Manuscripts presenting clinical research related to steroids, steroid drug development, comparative endocrinology of steroid hormones, investigations on the mechanism of steroid action and steroid chemistry are all appropriate for submission for peer review. STEROIDS publishes both original research and timely reviews. For details concerning the preparation of manuscripts see Instructions to Authors, which is published in each issue of the journal.