A novel approach for lymphatic organoid embedding: eosin pre-staining and agarose pre-embedding.

Adenoid organoids, as the primary immune barrier of the airway, provide valuable models for studying lymphatic tissue function, but their histological processing remains challenging due to their fragile structure and lack of adhesion. Here, we introduce a novel approach that combines eosin pre-staining with agarose pre-embedding to enhance visibility and structural integrity during paraffin embedding. This method simplifies sectioning and improves the quality of hematoxylin and eosin (HE) and immunofluorescence (IF) staining, yielding clear and stable signals. By addressing key limitations in lymphatic organoid processing, this technique provides a reliable solution for histological and IF studies, facilitating future research on adenoid organoids.